We narrowed to 5,093 results for: puc

1. pUC57-mini v2-HF24_F6

   Plasmid

   #195720

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#6 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pUC57-mini v2-HF24_F15

   Plasmid

   #195729

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#15 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pUC57-mini v2-HF24_F17

   Plasmid

   #195731

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#17 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pUC57-mini v2-HF24_F19

   Plasmid

   #195733

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#19 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pUC57-mini v2-HF24_F2

   Plasmid

   #195716

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#2 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pUC57-mini v2-HF24_F11

   Plasmid

   #195725

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#11 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pUC57-mini v2-HF24_F16

   Plasmid

   #195730

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#16 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pUC57-mini v2-HF24_F18

   Plasmid

   #195732

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#18 of 24 fragment splits in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pUC57-mini v2-HF24_F1

   Plasmid

   #195715

   Purpose

   This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

   Depositor

   Eric Cantor

   Article

   Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

   Insert

   Fragment#1 of a 24 fragment split in lacI/lacZ cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pUC57-mini v2-HF24_F9

    Plasmid

    #195723

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#9 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    Feb. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pUC57-mini v2-HF24_F23

    Plasmid

    #195737

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#23 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    Feb. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pUC57-mini v2-HF24_F21

    Plasmid

    #195735

    Purpose

    This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Insert

    Fragment#21 of 24 fragment splits in lacI/lacZ cassette
    

    Use

    Synthetic Biology

    Available Since

    Feb. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pUCP20 PilB113*Q119A

    Plasmid

    #162867

    Purpose

    Expression of PilB Amber with a C-terminal histag

    Depositor

    Katrina Forest

    Insert

    PilB
    

    Tags

    Histidine tag (H6x)

    Expression

    Bacterial

    Mutation

    Q113Amber, Q119A

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUCP20 PilB113*L197D

    Plasmid

    #162868

    Purpose

    Expression of PilB Amber with a C-terminal histag

    Depositor

    Katrina Forest

    Insert

    PilB
    

    Tags

    Histidine tag (H6x)

    Expression

    Bacterial

    Mutation

    Q113Amber, L193D

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC19_HR-BirA-FLAG_Ago2

    Plasmid

    #170918

    Purpose

    Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago2

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    BirA-FLAG
    

    Expression

    Mammalian

    Promoter

    lac

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19_HR-BirA-FLAG_Ago1

    Plasmid

    #170919

    Purpose

    Encodes BirA-FLAG with left and right homology arms for endogenous tagging of Ago1

    Depositor

    Constance Ciaudo

    Article

    Muller et al Life Sci Alliance. 2022 Mar 2;5(6). pii:

    Insert

    BirA-FLAG
    

    Expression

    Mammalian

    Promoter

    lac

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Rp51a_10bp 5'SS_No BP or pBP_pUC18

    Plasmid

    #100989

    Purpose

    Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

    Plasmid

    #100990

    Purpose

    Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. tagRFP-IMBB4-pUC

    Plasmid

    #170769

    Purpose

    test plasmid for 5RM assembly, encodes fluorescent red protein

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Insert

    tagRFP
    

    Promoter

    none

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUC19-SpRYgest_targetplasmid (KAC833)

    Plasmid

    #181748

    Purpose

    pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Type

    Empty backbone

    Use

    Substrate plasmid

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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