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YY120: pPB_TRE::GEMINI(B)-p2A-GEMINI(A)

Plasmid

#228881

Purpose

PiggyBac plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a doxycycline-inducible promotor.

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

Building blocks of GEMINI for intracellular assembly

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-P405A/P531A-pcDNA3

Plasmid

#18956

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

Plasmid

#170829

Purpose

PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d CLCN3 gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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APEX2-SOPP3-TM

Plasmid

#229077

Purpose

Expresses APEX2-SOPP3 in the cell surface

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the cell surface (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pYLsgRNA-AtU6-29

Plasmid

#66203

Purpose

cloning of sgRNAs for expression in plants

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Type

Empty backbone

Use

Cloning vector

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ10539

Plasmid

#239270

Purpose

Expresses PYL1-DDX6 for recruiting RNA to the p-body via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PYL1-BFP-DDX6 (DDX6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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YY105: pPB_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

Plasmid

#228882

Purpose

PiggyBac plasmid expressing the HaloTag-GEMINI(A) under the control of a doxycycline-inducible promotor, and rtTA3 and PuroR under the control of a UbC promoter.

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

HaloTag-fused GEMINI(A)

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA4-NHK-MYC-His6

Plasmid

#185350

Purpose

Assessing of ubiquitination in cells

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

a1-antitrypsin Hong Kong Null mutant

Tags

Myc, 6xHis

Expression

Mammalian

Mutation

NHK mutant

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCART0002_pIVTRup(BsmBI)_CBE4max-SpRY

Plasmid

#242540

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CBE4max-SpRY (SpRY-CBE)

Use

CRISPR

Mutation

D10A

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

Plasmid

#239974

Purpose

PiggyBac plasmid for inducible PEmax expression and Puromycin selection

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

Plasmid

#239972

Purpose

Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-MYO1D

Plasmid

#125125

Purpose

Expresses human FLAG-MYO1D

Depositor

Bruce Beutler

Article

McAlpine et al Dis Model Mech. 2018 Sep 27;11(9). pii:

Insert

MYO1D (MYO1D Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-hMALAT1

Plasmid

#118580

Purpose

to overexpress human MALAT1 in mammalian cells

Depositor

Jianjun Zhao

Article

Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

Insert

MALAT1 (MALAT1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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EFS-SpdCas9

Plasmid

#210703

Purpose

This plasmid express EFS promoter driven human codon optimized SpdCas9

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

S. Pyogenes dCas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a core promoter

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mNeptune2.5

Plasmid

#51310

Purpose

Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

Depositor

Michael Lin

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

mNeptune2.5

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only

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IKK-2 S177E S181E

Plasmid

#11105

Purpose

Mammalian expression of constitutively active human IKK-2

Depositor

Anjana Rao

Article

Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

Insert

IKK-2 (IKBKB Human)

Tags

FLAG

Expression

Mammalian

Mutation

S177E and S181E, constitutively active

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pcDNA3

Plasmid

#18955

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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PB-3-FT-ENGRAM

Plasmid

#179158

Purpose

piggyBac plasmid for 3'-FT ENGRAM recorder

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Insert

Csy4

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

minP

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AID-NG-CBE

Plasmid

#227416

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

AID-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-APEX2-SOPP3-3xNLS

Plasmid

#229074

Purpose

expresses APEX2-SOPP3 in the mammalian nucleus

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the mammalian nucleus (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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