We narrowed to 13,600 results for: sequence

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AAV-CMV-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

Plasmid

#177342

Purpose

AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence

Depositor

Naoki Yamamoto

Article

Yamamoto et al Research Square [Preprint] 2021. doi: 10

Insert

dead hSaCas9

Use

AAV and CRISPR

Tags

3x GCN4 peptide, 3xHA, and NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pECNUS2

Plasmid

#184885

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pECNUS3

Plasmid

#184886

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pECNUS1

Plasmid

#184884

Purpose

ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

Depositor

Eunyoung Chae

Article

Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

RPS5A

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pWR63(YUM1)

Plasmid

#203336

Purpose

episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
CaCas9
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
THD3 promoter (S. stipitis)
tRNA-sgRNA(SapI)-HDV
ADH1 terminator (S. stipitis)
YUM1-targeting sequence

Use

CRISPR

Expression

Yeast

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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AA375

Plasmid

#216054

Purpose

Fragmid fragment: (C' terminus) HIV nuclear export sequence; HA tag

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

HIV-NES_v1.1; HIV-NES_v1.2; 3xHA_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLC045 - pL0_CrGLK (CDS1ns)

Plasmid

#203904

Purpose

MoClo compatible L0 plasmid containing the CrGLK coding sequence, lacking a stop codon to allow for C-terminal fusions.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK (without a stop codon)

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLC047 - pL2_p2x35s-CrGLK-tMAS

Plasmid

#203906

Purpose

Overexpression of the CrGLK coding sequence from C. roseus in a plant binary vector for Agrobacterium-mediated transformation.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

CrGLK

Expression

Plant

Mutation

Silent mutation of BpiI or BsaI cut site for MoCl…

Promoter

CaMV 2x35S

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

Plasmid

#216378

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

HA signal sequence-FLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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FENEF-Sensor

Plasmid

#208930

Purpose

in vitro p38-Sensor for luminescent detection of p38-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

FENEF sequence from human ATF2 with phospho site (ATF2 Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_1

Plasmid

#210625

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_2

Plasmid

#210622

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_1

Plasmid

#210621

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_4

Plasmid

#210624

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_-60_Enh_3

Plasmid

#210623

Purpose

gRNA target sequences for TAL1 -60 Enhancer

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 -60 Enhancer gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_2

Plasmid

#210626

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_3

Plasmid

#210627

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pSPgRNA_Ex3_4

Plasmid

#210628

Purpose

gRNA target sequences for TAL1 Exon 3

Depositor

Maayan Salton

Article

Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

Insert

TAL1 Exon 3 gRNA (TAL1 Human)

Use

CRISPR

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

Plasmid

#207136

Purpose

DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)

Expression

Mammalian

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pet28b_His_RAD23A-UBA1

Plasmid

#201549

Purpose

Expresses UBA1 of human RAD23A in E. coli

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog A (RAD23A Human)

Tags

6x His

Expression

Bacterial

Mutation

Expresses amino acids 160-205 of DNA polymerase i…

Promoter

T7

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A695Q

Plasmid

#207368

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A695Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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B-HeFSpCas9-A926Q

Plasmid

#207370

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A926Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-E526K

Plasmid

#207365

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-E526K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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B-evoSpCas9-V495M

Plasmid

#207363

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-V495M

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Y515N, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A1060R

Plasmid

#207372

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1060R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A1003K

Plasmid

#207371

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1003K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A848K

Plasmid

#207369

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A848K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A661R

Plasmid

#207367

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-evoSpCas9-Q661R

Plasmid

#207366

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-Q661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, K526E, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-evoSpCas9-N515Y

Plasmid

#207364

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-N515Y

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge