We narrowed to 13,190 results for: CAR

1. pCMV4a-SIRT2-Flag

   Plasmid

   #102623

   Purpose

   Expresses full length SIRT2 isoform 1 in mammalian cells

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   Sirtuin 2 (SIRT2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-Neo-Bam p53 R249S

   Plasmid

   #16438

   Depositor

   Bert Vogelstein

   Article

   Baker et al Science. 1990 Aug 24. 249(4971):912-5.

   Insert

   p53 R249S (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   R249S

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR-JNKKTRClover

   Plasmid

   #59139

   Purpose

   ORF encoding JNK KTR mClover flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
   

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Oct4-IRES-eGFP-PGK-Neo

   Plasmid

   #48681

   Purpose

   For knock-in donor vector by CRISPR/CAS system

   Depositor

   Rudolf Jaenisch

   Article

   Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p2xFLAGhYAP1

   Plasmid

   #17791

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Tags

   2xFlag

   Expression

   Mammalian

   Available Since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRK5-FLAG-Sestrin2

   Plasmid

   #72595

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN2 (SESN2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCRISPR-Cre stuffer v4

   Plasmid

   #158033

   Purpose

   Lentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-FAB(mito-LD)

   Plasmid

   #214421

   Purpose

   Detection of mito-lipid droplet contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspAN-3xHA-V5-mito_IRES_CFAST-20nm-Halo-6xHp
   

   Use

   Lentiviral

   Tags

   CFAST-Halo and RspAN-3XHA-V5

   Promoter

   CMV

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Mito-CanlonicSF/pCMV-myc-mito

   Plasmid

   #224465

   Purpose

   To explore mitochondrial lactate dynamics

   Depositor

   Alejandro San Martín

   Article

   Rauseo et al bioRxiv 2024.07.19.604361

   Insert

   CanlonicSF
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132411

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRfxCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R239A; H244A; R858A; H863A

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. His-MBP_G4G6 in pRSF

    Plasmid

    #188454

    Purpose

    Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    G4G6 (GSN Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p3HM1Luc4

    Plasmid

    #236277

    Purpose

    Mammalian expression of human muscarinic M1 receptor; N-terminal HA tag, C-terminal Rluc8

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

    Insert

    muscarinic M1 receptor (CHRM1 Human)
    

    Tags

    3xHA and Rluc8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK-caCas9-NatMX-Neut5L-Leu2 drive

    Plasmid

    #89578

    Purpose

    caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Insert

    Leu2 gene drive
    

    Expression

    Yeast

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-GGA1

    Plasmid

    #178459

    Purpose

    Expresses GGA1 in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Puertollano et al Mol Biol Cell. 2003 Apr;14(4):1545-57. d

    Insert

    GGA1 (GGA1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSP64-hKCNE1

    Plasmid

    #53049

    Purpose

    Expresses beta subunit for IKs channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
    

    Use

    Xenopus oocyte expression

    Promoter

    SP6

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-p38KTRClover

    Plasmid

    #59142

    Purpose

    ORF encoding p38 KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5-HA-Sestrin2

    Plasmid

    #72593

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN2 (SESN2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pGEX-4T-1 3xFlag-MAPKAPK2

    Plasmid

    #82720

    Purpose

    Expresses human MAPKAPK2

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MAPKAPK2 (MAPKAPK2 Human)
    

    Tags

    3xFLAG and GST

    Expression

    Bacterial

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

19. T/Brachyury-eGFP Rex Neo

    Plasmid

    #21222

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. eSpCas9(1.1)-Puro

    Plasmid

    #117686

    Purpose

    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

    Depositor

    Luc Leyns

    Article

    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

    Insert

    eSpCas9(1.1)
    

    Use

    CRISPR

    Tags

    PuroR (R166H)

    Expression

    Mammalian

    Mutation

    K848A, K1003A, R1060A

    Promoter

    U6

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge