We narrowed to 10,853 results for: ENA

1. pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

   Plasmid

   #187337

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Michael Bassik, Lacra Bintu

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-AIO-M11-gRNA-EFS-NMS-SadCas9

   Plasmid

   #210710

   Purpose

   This Plasmid express M11 promoter driven SadCas9 specific gRNA and EFS promoter driven NMS transactivation module fused to SadCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   SadCas9 specific gRNA and NMS fused to N-terminus of SadCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/N580A

   Promoter

   M11

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRIP-SFFV-mtagBFP-2A STING V155M 368STOP

   Plasmid

   #102596

   Purpose

   Lentivector to express STING V155M delta 368 and mTagBFP

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M delta 368

   Promoter

   SFFV

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. EXO1B-AcGFP

   Plasmid

   #215125

   Purpose

   Expresses EXO1B-AcGFP in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   EXO1B (EXO1 Human)
   

   Use

   Retroviral

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. B270 + SMARCAL1 sgSTOP

   Plasmid

   #100717

   Purpose

   B270 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) in combination with ATP1A1 sgSTOP

   Depositor

   Alberto Ciccia

   Article

   Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

   Insert

   sgSTOP targeting SMARCAL1 (cloned using BbsI) and ATP1A1 (SMARCAL1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Cyclin F

   Plasmid

   #215054

   Purpose

   Expresses EGFP-Cyclin F in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCNF (CCNF Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Anti-VDAC1 [N152B/23R]

   Plasmid

   #177502

   Purpose

   Mammalian Expression Plasmid of anti-VDAC1 (Human). Derived from hybridoma N152B/23.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-VDAC1 (Homo sapiens) recombinant mouse monoclonal antibody (VDAC1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCerulean-PCNA-HygroR

   Plasmid

   #215064

   Purpose

   Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Anti-Pan-Synapsin [L125/129R]

   Plasmid

   #177472

   Purpose

   Mammalian Expression Plasmid of anti-Pan-Synapsin (Human). Derived from hybridoma L125/129.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Pan-Synapsin (Homo sapiens) recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

    Plasmid

    #100716

    Purpose

    B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting ATP1A1 (cloned using BsmBI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1.shCDK8

    Plasmid

    #19760

    Depositor

    William Hahn

    Article

    Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

    Insert

    small hairpin RNA against CDK8 (CDK8 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCDH-CB1-HIF2a-GFP-T2A-Puro

    Plasmid

    #71708

    Purpose

    Lentiviral expression of HIF2A-GFP

    Depositor

    Eric Jonasch, Xiande Liu

    Article

    Liu et al Oncogene. 2015 May 7;34(19):2450-60. doi

    Insert

    HIF2A (EPAS1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. SspB-HaloTag-DHPH-ITSN1

    Plasmid

    #176113

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75240

    Purpose

    CRISPR/Cas9 plasmid against human Ikaros

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSS02:cyt-Peredox-mCherry_DS

    Plasmid

    #161747

    Purpose

    cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

    Depositor

    Markus Schwarzländer

    Article

    Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

    Insert

    cyt-Peredox-mCherry_DS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    amino acid substitution D115S in first T-Rex doma…

    Promoter

    Ubiquitin 10

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT218 lenti pEF-rTetR(SE-G72P)-3XFLAG-VP64-T2A-mCherry-BSD-WPRE

    Plasmid

    #188758

    Purpose

    Lentiviral backbone containing VP64 as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    VP64
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1a

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lentiviral click editor (CE1) plasmid - LTR-pEF1a-PCV2-nCas9-EcKlenow (DRR921)

    Plasmid

    #217798

    Purpose

    Lentiviral plasmid to produce the prototypical CE1 construct (PCV2-nCas9-EcKlenow), expressed from the EF1a promoter

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR and Lentiviral

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    EF1a

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLNCX2-NGN2-IRES-GFP-T2A-Sox11

    Plasmid

    #90213

    Purpose

    To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

    Depositor

    Chun-Li Zhang

    Article

    Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

    Insert

    NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Mouse, Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid

    #208943

    Purpose

    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75241

    Purpose

    CRISPR/Cas9 plasmid against human Ikaros

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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