We narrowed to 9,260 results for: pho;

1. pGEX6P1 p37

   Plasmid

   #169015

   Purpose

   Expresses GST-p37 (human, codon optimized for expression in bacteria)

   Depositor

   Hemmo Meyer

   Article

   Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

   Insert

   p37 (UBXN2B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   sequence was codon optimized for expression in E.…

   Promoter

   tac

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDuet-1 IntS6 AA 1035-1284

   Plasmid

   #196904

   Purpose

   Expresses His-tagged Drosophila IntS6 amino acids 1035-1284

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 6 (IntS6 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-TetO-Tfap2c

   Plasmid

   #128826

   Purpose

   dox-dependent expression of mouse Tfap2c

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

   Insert

   Tfap2c (Tfap2c Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCerulean3-MAPTau-C-10

   Plasmid

   #55431

   Purpose

   Localization: Microtubules, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   MAPTau (MAPT Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-V5-PTPN14-del-C

   Plasmid

   #61005

   Purpose

   Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

   Depositor

   Jianmin Zhang

   Article

   Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

   Insert

   PTPN14 delete C (PTPN14 Human)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 934-1187

   Promoter

   CMV

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pYFP-NGAT(A193T,N194Y)

   Plasmid

   #11388

   Depositor

   Joel Swanson

   Article

   Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

   Insert

   golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Changed Alanine 193 to Threonine, and Asparagine …

   Promoter

   Available since

   March 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET-pr-mKikGR1 (mKikGR1-V69T)

   Plasmid

   #99227

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mKikGR1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   monomeric variant with mutation V69T (Eos notatio…

   Promoter

   T7

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-1 IntS11 AA 300-597

   Plasmid

   #199329

   Purpose

   Expresses His-tagged Drosophila IntS11 amino acids 300-597

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 11 (IntS11 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 300-597 only

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmirGLO-Bmpr2-1719

   Plasmid

   #49379

   Purpose

   Insert is a fragment of the Bmpr2 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

   Depositor

   Martine Roussel

   Article

   Murphy et al Cancer Res. 2013 Oct 21.

   Insert

   Bmpr2 (BMPR2 Human, Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Human phosphoglycerate kinase (PGK) promoter

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-LplA(AAG)

    Plasmid

    #61307

    Purpose

    Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression

    Depositor

    Alice Ting

    Article

    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

    Insert

    E. coli lipoic acid ligase
    

    Use

    Tags

    6xHis, Flag, T7 tag, and Xpress epitope

    Expression

    Mammalian

    Mutation

    E20A, F147A, H149G

    Promoter

    CMV

    Available since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. LENTICRISPR-TPI1_sgRNA1

    Plasmid

    #201626

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    TPI1 (TPI1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. LENTICRISPR-TPI1_sgRNA2

    Plasmid

    #201627

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    TPI1 (TPI1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMX-SHP1C-ires-hCD8

    Plasmid

    #183720

    Purpose

    Expresses the myc-tagged catalytic domains of wild-type SHP-1.

    Depositor

    Takeshi Tsubata

    Article

    Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

    Insert

    SHP-1
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    catalitic domain alone

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT3035

    Plasmid

    #122543

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a nuclear lamin localized gene (lmn-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_lmn-1::CemOrange2 (lmn-1 Synthetic, Nematode)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pnhx-2

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mCherry-TSC2(AA)-NLS

    Plasmid

    #192443

    Purpose

    Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

    Promoter

    CMV

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5FRT/TO-PLAA-Strep-HA

    Plasmid

    #113484

    Purpose

    Expression of human PLAA with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    PLAA (PLAA Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. YFP-Stim1-ΔCTD

    Plasmid

    #186621

    Purpose

    Encodes Stim1 with C-terminal domain deletion fused to YFP

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Stromal Interaction Molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain 601 to 685

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn1 CaRhAC T2A tDimer

    Plasmid

    #101722

    Purpose

    - humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neurons

    Depositor

    Thomas Oertner

    Article

    Scheib et al Nat Commun. 2018 May 24;9(1):2046. doi:

    Inserts

    CatRhAC
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E497K,C569D

    Promoter

    human Synapsin1 promotor and ribosomal skip seque…

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. tet-pLKO shMKP5.v2 puro

    Plasmid

    #87793

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. tet-pLKO shMKP5.v1 puro

    Plasmid

    #87792

    Purpose

    Expresses an inducible short hairpin targeting human MKP5 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP5 (DUSP10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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