Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 28,411 results for: REP

Showing: 6881 - 6900 of 28411 results

  1. pGS1512

    Plasmid
    #63788
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eVenus Strep-tag II followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eVenus-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    eVenus-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63788-plasmid-map-sequence-id-106476

    Enlarge

  2. pGS1312

    Plasmid
    #63786
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Strep-tag II followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCFP-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    eCFP-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63786-plasmid-map-sequence-id-106475

    Enlarge

  3. p3015b

    Plasmid
    #209317
    Purpose
    Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.
    Depositor
    Thomas Hooven
    Article
    Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1
    Insert
    Group B Streptococcus-compatible single guide RNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Xyl/tet promoter that is constitutively active
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    209317-plasmid-map-sequence-id-435309

    Enlarge

  5. pHDR-USR-SpCas9

    Plasmid
    #208829
    Purpose
    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.
    Depositor
    Zhiying Zhang
    Article
    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    208829-plasmid-map-sequence-id-419661

    Enlarge

  7. pGS1021

    Plasmid
    #59575
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    59575-plasmid-map-sequence-id-92981

    Enlarge

  9. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    59576-plasmid-map-sequence-id-92983

    Enlarge

  10. pGS1212

    Plasmid
    #63785
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eGFP-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63785-plasmid-map-sequence-id-106471

    Enlarge

  11. pGS1613

    Plasmid
    #63789
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    tDimer2-Strep-tag II followed by HIS5
    Use
    E. coli cloning vector
    Tags
    tDimer2-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63789-plasmid-map-sequence-id-110438

    Enlarge

  12. pGS1122

    Plasmid
    #63790
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Twin-Strep tag followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eSapphire-Twin-Strep tag
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63790-plasmid-map-sequence-id-106477

    Enlarge

  13. pGS1412

    Plasmid
    #63787
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCitrine-Strep-tag II followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    eCitrine-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    63787-plasmid-map-sequence-id-106472

    Enlarge

  14. pCRISPR-aBEST

    Plasmid
    #131464
    Purpose
    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Inserts
    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tipA
    Available since
    Nov. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    131464-plasmid-map-sequence-id-261686

    Enlarge

  16. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Expression
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

    Enlarge

  19. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid
    #60600
    Purpose
    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Article
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Inserts
    CRISPR Cas9 D10A
    puromycin resistance gene
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Changed Asp 10 to Ala, Codon usage optimized for …
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    60600-plasmid-map-sequence-id-97110

    Enlarge

  20. pLY164

    Plasmid
    #184140
    Purpose
    Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:
    Inserts
    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    Use
    Synthetic Biology
    Tags
    ASV tag and λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Pcon, PpspA-R3, PrhaB, and Ptet
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184140-plasmid-map-sequence-id-378329

    Enlarge

Showing: 6881 - 6900 of 28411 results