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pT7-EGFP-C1-HsNanos1_P

Plasmid

#147201

Purpose

Mammalian Expression of HsNanos1

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1 (NANOS1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNanos2_P

Plasmid

#147202

Purpose

Mammalian Expression of HsNanos2

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2 (NANOS2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1-Dm7SL-RNA-long_M

Plasmid

#146891

Purpose

Insect Expression of Dm7SL-RNA-long

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

Dm7SL-RNA-long (7SLRNA:CR32864 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1-Dm7SL-RNA-short_M

Plasmid

#146892

Purpose

Insect Expression of Dm7SL-RNA-short

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

Dm7SL-RNA-short (7SLRNA:CR32864 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-417_M

Plasmid

#146895

Purpose

Insect Expression of DmCup_1-417

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-417 (cup Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_1-779_M

Plasmid

#146902

Purpose

Insect Expression of DmCup_1-779

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_1-779 (cup Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup_418-1132_M

Plasmid

#146906

Purpose

Insect Expression of DmCup_418-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_418-1132 (cup Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCup-L379AL383A_M

Plasmid

#146912

Purpose

Insect Expression of DmCup-L379AL383A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-L379AL383A (cup Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-CS-DmCup_311-440_M

Plasmid

#146913

Purpose

Bacterial Expression of DmCup_311-440

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_311-440 (cup Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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SL-TREAT

Plasmid

#196918

Purpose

The TREAT reporter containing cap-proximal GC-rich stemloop

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGB-05-06-GST-mCherry-ATG14

Plasmid

#187988

Purpose

Plasmid for the protein expression of GST-mCherry-ATG14 in a bacterial and insect expression system. Internal_Reference: SMC1322

Depositor

Sascha Martens

Insert

ATG14 (ATG14 Human)

Tags

GST-mCherry

Expression

Insect and Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH059

Plasmid

#186641

Purpose

aadA.co-TaadA-PrbcL-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-PrbcL-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH140

Plasmid

#186643

Purpose

aadA.co-TaadA-PJ23111-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-PJ23111-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH141

Plasmid

#186644

Purpose

aadA.co-TaadA-PJ23100-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-PJ23100-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pJMH142

Plasmid

#186645

Purpose

aadA.co-TaadA-PJ23107-eYFP.co between homology arms for pilA

Depositor

Adam Arkin

Article

Hilzinger et al Biotechnol Bioeng. 2025 Jan;122(1):44-52

Insert

aadA.co-TaadA-PJ23107-eYFP.co

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos1_X

Plasmid

#147954

Purpose

Mammalian Expression of HsNanos1

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1 (NANOS1 Human)

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos2_X

Plasmid

#147960

Purpose

Mammalian Expression of HsNanos2

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2 (NANOS2 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-matDYK

Plasmid

#115258

Purpose

For mammalian expression of the human NODAL open reading frame with an internal DYK tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODAL-T7TS

Plasmid

#115250

Purpose

For in vitro transcription of human NODAL open reading frame RNA

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-1-Tbx5-site

Plasmid

#177832

Purpose

Luciferase reporter for mouse Cxcl1 with one Tbx5 consensus binding site

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Promoter

Cxcl1

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCxcl1-Firefly-2-Tbx5-sites

Plasmid

#177833

Purpose

Luciferase reporter for mouse Cxcl1 with two Tbx5 consensus binding sites

Depositor

Alexander Ruthenburg

Article

Hall et al bioRxiv 2021.12.23.474040

Insert

Luciferase

Use

Luciferase

Promoter

Cxcl1

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-TalO8 (Blank-CoTrIP)

Plasmid

#178303

Purpose

Blank CoTrIP plasmid

Depositor

Brian Zid

Article

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Type

Empty backbone

Tags

NA

Expression

Yeast

Promoter

NA

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIN1_GGGSx3_mScarlet

Plasmid

#182936

Purpose

C-terminal mScarlet with flexible linker (GGGGS)x3

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Type

Empty backbone

Expression

Worm

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-ssIFNA2

Plasmid

#183469

Purpose

Expression of the Salmo salar interferon ifna2

Depositor

Bertrand Collet

Article

Collins et al Fish Shellfish Immunol. 2014 Oct;40(2):6

Insert

Salmo salar interferon alpha 2 precursor

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-foxd4l1.1-myc

Plasmid

#185495

Purpose

Expression of Xenopus laevis foxd4

Depositor

Sally Moody

Article

Yan et al Dev Biol. 2009 May 1;329(1):80-95. doi:

Insert

foxd4l1.1

Use

In vitro transcription

Tags

myc

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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Sp(AU)sgRNA-mKO2

Plasmid

#184443

Purpose

sgRNA with AU flip (sgRNA2.0), mKO2 as selection marker

Depositor

Rhys Allan

Article

Chan et al Immunol Cell Biol. 2020 Sep 24. doi: 10.

Insert

sgRNA with AU flip, mKO2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo-ssIFNG

Plasmid

#183470

Purpose

Expression of the Salmo salar gamma interferon

Depositor

Bertrand Collet

Article

Collins et al Fish Shellfish Immunol. 2014 Oct;40(2):6

Insert

Salmo salar interferon gamma precursor

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCup_771-1132_C

Plasmid

#146030

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCup_771-1132_C

Plasmid

#146034

Purpose

Insect Expression of DmCup_771-1132

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup_771-1132 (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCup-Y342AL347A_C

Plasmid

#146035

Purpose

Insect Expression of DmCup-Y342AL347A

Depositor

Elisa Izaurralde

Article

Igreja et al Genes Dev. 2011 Sep 15;25(18):1955-67. d

Insert

DmCup-Y342AL347A (cup Fly)

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge