We narrowed to 11,684 results for: cel.2

1. pMTS_mScarlet_N1

   Plasmid

   #85057

   Purpose

   In vivo visualization of the mitochondria (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   MTS (COX8A Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   aa1-29

   Promoter

   CMV

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-PE

   Plasmid

   #162796

   Purpose

   All-in-one prime editor piggyBac transposon

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRB-GFP-Gab2(Y604F/Y634F)

   Plasmid

   #188659

   Purpose

   FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   GRB associated binding protein 2 (Gab2 Mouse)
   

   Use

   Tags

   FRB and GFP

   Expression

   Mammalian

   Mutation

   Y604F and Y634F

   Promoter

   CMV

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1-PHD2

   Plasmid

   #223552

   Purpose

   Lentivirus transfer plasmid for expression of full length human PHD2

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. (114) pcDNA3.1-GFP-Flag-JunB-EPEA

   Plasmid

   #160743

   Purpose

   Expresses a GFP tagged JunB with Flag and EPEA tags.

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   GFP-Flag-JunB-EPEA (JUN Human)
   

   Use

   Tags

   EPEA, Flag, and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. tetO-GATA6

   Plasmid

   #170688

   Purpose

   doxycycline-inducible overexpression of GATA6

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   GATA6 (GATA6 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUltraI-Tet3.0[TAA]

   Plasmid

   #164580

   Purpose

   Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Inserts

   Mb Tet3.0RS (R2-3)
   Mm tRNA[TAA]
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L170G, N311G, C313A

   Promoter

   ProK and TacI

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC_N_LMNA

   Plasmid

   #111688

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   LMNA (LMNA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pg-proPHD2-PHD2-EGFP

   Plasmid

   #87210

   Purpose

   PHD2-EGFP expressed under the control of the PHD2 promoter

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H313N

   Promoter

   PHD2

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-B-161;+80pCalb2

    Plasmid

    #66745

    Purpose

    luciferase reporter for Calb2 promoter (-161to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-161bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. Str-STIM1-NN_puromycin

    Plasmid

    #65310

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Use

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL3-B-264;+80pCalb2

    Plasmid

    #66746

    Purpose

    luciferase reporter for Calb2 promoter (-264 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-264bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-B-419;+80pCalb2

    Plasmid

    #66747

    Purpose

    luciferase reporter for Calb2 promoter (-419 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-419bp+80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. tetO-SRF

    Plasmid

    #170704

    Purpose

    doxycycline-inducible overexpression of SRF

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SRF (SRF Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid

    #48227

    Purpose

    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    

    Use

    CRISPR

    Tags

    2A-neo, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. tetO-ETS2

    Plasmid

    #170685

    Purpose

    doxycycline-inducible overexpression of ETS2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ETS2 (ETS2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMX-IY HA-GBRPL2

    Plasmid

    #89298

    Purpose

    For expression of the mammalian Atg8 protein GABARAPL2 tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    GABARAPL2 (GABARAPL2 Human)
    

    Use

    Retroviral

    Tags

    HA and IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5 FRT TO Myc ArB WT RNAiR

    Plasmid

    #59806

    Purpose

    Allows the integration of Myc ArB in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora B (AURKB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    RNAi resistant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMTS_mScarlet-H_N1

    Plasmid

    #85058

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    MTS (COX8A Human)
    

    Use

    Tags

    mScarlet-H

    Expression

    Mammalian

    Mutation

    aa1-29

    Promoter

    CMV

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. tetO-ESRRG

    Plasmid

    #170684

    Purpose

    doxycycline-inducible overexpression of ESRRG

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ESRRG (ESRRG Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKIF1C-BioID2-HA

    Plasmid

    #130980

    Purpose

    Expression of HA-tagged KIF1C-BioID2

    Depositor

    Anne Straube

    Article

    Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

    Insert

    KIF1C-BioID2-HA (KIF1C Human)
    

    Use

    Tags

    BioID2-HA

    Expression

    Mammalian

    Mutation

    5 silent mutations that make this construct RNAi …

    Promoter

    CMV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. GFP-AuroraB L154G/H250Y

    Plasmid

    #108492

    Purpose

    expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Analog sensitive L154G/H250Y

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCR3-Flag-AuroraB KD (K106R)

    Plasmid

    #108488

    Purpose

    expression of Flag-AuroraB kinase death (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL3-B-838;+80pCalb2

    Plasmid

    #66750

    Purpose

    luciferase reporter for Calb2 promoter (-838 to +80, WT)

    Depositor

    Rolf Stahel, Emanuela Felley-Bosco

    Article

    Kresoja-Rakic et al Oncotarget. 2016 Feb 1. doi: 10.18632/on

    Insert

    Calb2 promoter (-838bp +80) (CALB2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Calb2

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EBFP2-UtrCH

    Plasmid

    #124656

    Purpose

    F-actin marker. EBFP2 is fused to the calponin homology domain of utrophin (UtrCH).

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    Utrophin (UTRN Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5 TO FRT Myc ArB K106R

    Plasmid

    #59807

    Purpose

    Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora B (AURKB Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K106R, kinase mutant

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMX-IY GBRPL2

    Plasmid

    #89295

    Purpose

    For expression of the mammalian Atg8 protein GABARAPL2 without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    GABARAPL2 (GABARAPL2 Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-RanV19 E1RA2

    Plasmid

    #118317

    Purpose

    Expression of human Ran

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Inserts

    RanV19 (RAN Human)
    EPac1RA2 (RAPGEF3, RAPGEF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    in-frame insertion of RanV19 into GFPEpac1RA2 and…

    Promoter

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GST-ENLYFQC-PNT(46-138)-His6

    Plasmid

    #42919

    Depositor

    Barbara Imperiali

    Article

    Lukovic et al Angew Chem Int Ed Engl. 2009;48(37):6828

    Insert

    Ets-1 PNT domain (46-138) (Ets1 Mouse)
    

    Use

    Tags

    6xHis, GST, and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Contains the PNT domain (Serine 46 through Lysine…

    Promoter

    Ptac

    Available since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pIS1-mutant RhoA 3'UTR

    Plasmid

    #26090

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    mutant RhoA 3'UTR (RHOA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Both miR-31 binding sites in 3’ UTR mutagenized. …

    Promoter

    Available since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only