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pAGM9121_Sce-Invertase 2 SP

Plasmid

#153493

Purpose

S. cerevisiae Invertase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Invertase 2 SP

Use

Synthetic Biology

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pC0.007

Plasmid

#119671

Purpose

Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

Depositor

Alistair McCormick

Publication

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Pcpc560-Dx2

Use

Synthetic Biology

Mutation

bp 89 T to C, bp 187 C to A, Duplicated downstrea…

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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brr2-C520R pRS313

Plasmid

#111412

Purpose

brr2-C520R (endogenous promoter)

Depositor

Christine Guthrie

Publication

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Expression

Yeast

Mutation

C520R aa mutation

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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brr2-N1104A pRS313

Plasmid

#111414

Purpose

brr2-N1104A (endogenous promoter)

Depositor

Christine Guthrie

Publication

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Expression

Yeast

Mutation

N1104A aa mutation

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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brr2-R1107L pRS313

Plasmid

#111416

Purpose

brr2-R1107L (endogenous promoter)

Depositor

Christine Guthrie

Publication

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Expression

Yeast

Mutation

R1107L aa mutation

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-RFP-miR-30 backbone

Plasmid

#97147

Purpose

As a control of miRNA expression

Depositor

Qing Deng

Publication

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC

Plasmid

#101828

Purpose

drives expression of kaiBC from an rpaA-independent promoter

Depositor

Erin O'Shea

Publication

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kanamycin casette and p0050 promoter

Use

Cloning vector

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90285

Purpose

BB2 with split pyrG marker for homologous integration with linker AB (negative control)

Depositor

Michael Sauer

Publication

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90286

Purpose

BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)

Depositor

Michael Sauer

Publication

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90287

Purpose

BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

Depositor

Michael Sauer

Publication

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90288

Purpose

BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

Depositor

Michael Sauer

Publication

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Publication

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

pU6

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(KTF)-HA no-pA

Plasmid

#82596

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

W2101F

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDS2-aurZ

Plasmid

#70151

Purpose

Fusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection marker

Depositor

Morten Sommer

Publication

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

aurZ

Expression

Yeast

Promoter

Tef

Available since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pPK1E-2+t

Plasmid

#48148

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Publication

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pPK1E-2

Plasmid

#48146

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Publication

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pPK1E-1

Plasmid

#48145

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Publication

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pSA40

Plasmid

#35114

Depositor

John McCusker

Publication

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

lys5 flanked by loxp

Use

Yeast replicating

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-F1-GTG-F2-GGT-F3-GAA

Plasmid

#30707

Depositor

Nathan Lawson, Scot Wolfe

Publication

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GTG, F2 GGT, F3 GAA

Use

Mrna production

Tags

HA

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
PrbC5

Plasmid

#24787

Depositor

David Baker

Publication

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only