We narrowed to 11,713 results for: NSI

1. pLX304_zeo

   Plasmid

   #160092

   Purpose

   Lentiviral vector for mammalian gene expression

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CaMKIV-dCT

   Plasmid

   #126422

   Purpose

   Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)

   Depositor

   Douglas Black

   Article

   Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi

   Insert

   Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSW646 - pML1>>

   Plasmid

   #115995

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CAG.Flex.NES-jRGECO1b.WPRE.SV40

   Plasmid

   #100855

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   jRGECO1b
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer20-Blast-CDT1-HA

   Plasmid

   #109335

   Purpose

   virus packaged, doxycyline inducible expression of Cdt1

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Insert

   Cdt1 (CDT1 Human)
   

   Use

   Lentiviral; Inducible expression

   Tags

   HA, His, Strep

   Expression

   Mammalian

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST40-2XHA-Src-Y419F

   Plasmid

   #140316

   Purpose

   Expresses HA-tagged human Src-Y419F in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   Y419F

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_eGFP (open)

   Plasmid

   #160094

   Purpose

   Expresses eGFP in mammalian cells

   Depositor

   Rizwan Haq

   Article

   Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

   Insert

   eGFP
   

   Use

   Gateway entry vector

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-FLAG-MTOR-T1977R

   Plasmid

   #69011

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-T1977R (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Threonine 1977 to Arginine

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLentiCRISPRv2 sgBLM10

   Plasmid

   #127643

   Purpose

   Knock-out of human BLM

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   IRF3 sgRNA (IRF3 Human)
   

   Use

   Lentiviral

   Available Since

   July 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

    Plasmid

    #158952

    Purpose

    Express human RAD51(F129A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed phenylalanine 129 to alanine

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid

    #101300

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-509, 670-698, and 699-906 …

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-2XFL-Src-S4-KD

    Plasmid

    #140297

    Purpose

    Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CamKII-ArchT-GFP (PV2527)

    Plasmid

    #99039

    Purpose

    AAV-mediated expression of ArchT under the CamKII promoter

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    ArchT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_HCLS1-1/1

    Plasmid

    #91437

    Purpose

    Protein expression and purification of human SH3 domain construct HCLS1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    HCLS1-1/1 (HCLS1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CamKII-ArchT-GFP

    Plasmid

    #37807

    Purpose

    Viral expression of ArchT-GFP for optogenetic inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV; Adeno-associated virus

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMTTH+mEGFP-H1.4

    Plasmid

    #157799

    Purpose

    Expression of mEGFP and human linker histone H1.4 fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-H1.4 (H1-4 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    pTAC

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKD279.8

    Plasmid

    #173171

    Purpose

    Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

    Depositor

    Jeffrey Tabor

    Article

    Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

    Inserts

    SO_4388(REC)-PsdR(DBD) 137
    sfGFP
    

    Expression

    Bacterial

    Promoter

    J23108 and PpsdA110

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-ClipF-sesRNA-smV5-tTA2-W3SL

    Plasmid

    #192067

    Purpose

    Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for human FXOP2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-SUMO- flag-hDDI2-D252N_pET11a

    Plasmid

    #169123

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Mutation

    D252N

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-SUMO-flag-hDDI2_pET11a

    Plasmid

    #169121

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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