We narrowed to 11,713 results for: NSI

1. mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

   Plasmid

   #216763

   Purpose

   PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT
   

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSW505 - pOp4:3xGFP-NLS

   Plasmid

   #116005

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pOp4:B-dummy:3xGFP:linker-NLS:tUBQ10::SulfR
   

   Tags

   linker-NLS

   Expression

   Plant

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NSK026 CMV-TO-p53R248W (FLP-IN)

   Plasmid

   #191168

   Purpose

   Expression of human p53 mutant R248W in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   p53(R248W) (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   R248W

   Promoter

   CMV-TO

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSW648 - pREV>>

   Plasmid

   #115996

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Available Since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-FLAG-MTOR-F1888L

   Plasmid

   #69010

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-F1888L (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Phenylalanine 1888 to Leucine

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. H2B-mAvicFP1

   Plasmid

   #129511

   Purpose

   Expresses H2B-mAvicFP1 in mammalian cells (human histone 2B)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   H2B-mAvicFP1
   

   Expression

   Mammalian

   Mutation

   mAvicFP1 fused to the C terminus of human histone…

   Promoter

   CMV

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1-puro-irf3-shrna5

   Plasmid

   #127649

   Purpose

   Knock-down of human IRF3

   Depositor

   Nicolas Manel

   Article

   Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

   Insert

   IRF3 shRNA (IRF3 Human)
   

   Use

   Lentiviral

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MEIS1A-MIY

   Plasmid

   #21013

   Depositor

   Corey Largman

   Article

   Fischbach et al Blood. 2005 Feb 15. 105(4):1456-66.

   Insert

   MEIS1A (Meis1 Mouse)
   

   Use

   Retroviral

   Tags

   IRES and YFP

   Expression

   Mammalian

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pMSCV neo WT Akt1

   Plasmid

   #14549

   Depositor

   Joan Brugge

   Article

   Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mammalian

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pGL3-Tet2 promoter F

    Plasmid

    #63879

    Purpose

    Luciferase reporter plasmid used to study transcriptional control of murine Tet2

    Depositor

    Kian Peng Koh

    Article

    Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

    Insert

    Tet2 Promoter fragment F
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAS_4xMx PylT_FLAG-Mx PylRS YA

    Plasmid

    #140012

    Purpose

    Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y126A

    Promoter

    EF1

    Available Since

    June 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)

    Plasmid

    #61841

    Purpose

    mammalian expression of cyclin B1 non-degradable L45A mutant

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    cyclin B1 (CCNB1 Human)
    

    Tags

    2xSTREP and 3xFLAG

    Expression

    Mammalian

    Mutation

    L45A

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pNCST-mAvicFP1

    Plasmid

    #129509

    Purpose

    Expresses mAvicFP1 constitutively in E. coli (most strains)

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    mAvicFP1
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive (stationary phase) promote…

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV_hSyn1_AchLightR

    Plasmid

    #187463

    Purpose

    Expresses red acetylcholine indicator AchLightR in neuronal cells

    Depositor

    Tommaso Patriarchi

    Article

    Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

    Insert

    AchLightR
    

    Use

    AAV

    Tags

    Igκ leader

    Promoter

    human Synapsin-1

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MAC_C_COX41

    Plasmid

    #111664

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    COX41 (COX4I1 Human)
    

    Expression

    Mammalian

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT/TO-B2AR-APEX

    Plasmid

    #96848

    Purpose

    For expression of B2AR-APEX in mammalian cells

    Depositor

    Andrew Kruse

    Article

    Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

    Insert

    B2AR-APEX (ADRB2 Human)
    

    Expression

    Mammalian

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP-Orf9b L52D

    Plasmid

    #165129

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Orf9b L52D
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBp-FGFR3c-R248C

    Plasmid

    #45712

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR3 IIIc (FGFR3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    R248C

    Available Since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40

    Plasmid

    #100856

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Insert

    jRGECO1b
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2-FLAG-hEcto/Tif1g-CAmut

    Plasmid

    #20903

    Depositor

    Stefano Piccolo

    Article

    Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

    Insert

    Ectodermin (TRIM33 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Point mutagenesis of C125 and C128 to Alanine (RI…

    Available Since

    May 15, 2009

    Availability

    Academic Institutions and Nonprofits only