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We narrowed to 66,707 results for: nin

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Showing: 6941 - 6960 of 66707 results

  1. CA-Stat3-t2A-mCherry

    Plasmid
    #127540
    Purpose
    Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.
    Depositor
    Jennifer Mitchell
    Publication
    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Stat3 (Stat3 Mouse)
    Expression
    Mammalian
    Mutation
    A662C and N664C. Residues changed to Cys renders …
    Promoter
    CMV
    Available since
    July 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127540-plasmid-map-sequence-id-251258

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  3. pMM289

    Plasmid
    #46037
    Depositor
    Raymond Monnat
    Publication
    Swanson et al DNA Repair (Amst). 2004 May 4;3(5):475-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    WRN E84A/K577M (WRN Human)
    Use
    Tre, bidirectional promoter
    Tags
    5x myc
    Expression
    Mammalian
    Mutation
    E84A/K577M exonuclease and helicase-dead mutant; …
    Promoter
    CMV2
    Available since
    May 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    46037-plasmid-map-sequence-id-64899

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  6. pCAG-mEGFP-CaMKIIa (T286D)

    Plasmid
    #127391
    Purpose
    Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286D)
    Depositor
    Ryohei Yasuda
    Publication
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    changed Threonine 286 to Aspartic acid
    Promoter
    pCAG
    Available since
    Jan. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    127391-plasmid-map-sequence-id-250431

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  7. pSIN-TRE-EB3N-VVDfast-mCl3-iLID

    Plasmid
    #190169
    Purpose
    Doxycycline-inducible lentivirus vector to express EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60.
    Depositor
    Anna Akhmanova
    Publication
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Insert
    EB3N-VVDfast-mClover3-iLID (MAPRE3 Human, Synthetic)
    Use
    Lentiviral
    Tags
    -VVDfast-mClover3-iLID
    Mutation
    1-189 aa from EB3
    Promoter
    TRE
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190169-plasmid-map-sequence-id-373726

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  8. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Publication
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  9. yeast_DualReporter

    Plasmid
    #127546
    Purpose
    A dual reporter vector modified from Sharon et al (Nature Biotech, 2012) containing Ura3 and Nat1 selectable markers, a constant background RFP (TEF2::mCherry), and a variable YFP (pTpA+MCS::yEVenus).
    Depositor
    Aviv Regev
    Publication
    de Boer et al Nat Biotechnol. 2020 Jan;38(1):56-65. do
    Inserts
    bla
    mCherry
    URA3 (URA3 Budding Yeast)
    yEVenus
    Nat1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    TEF, TEF2, URA3, bla (E. coli), and pTpA+MCS
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127546-plasmid-map-sequence-id-251803

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  10. pDEST-CMV 3xFLAG-LC3A-GFP

    Plasmid
    #123106
    Purpose
    Expresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    Tags
    3xFLAG and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123106-plasmid-map-sequence-id-244007

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  13. pSLPB2B-FKBP12_F36V-CasRx-tagBFP-PGK-Blasticidin

    Plasmid
    #187952
    Purpose
    FKBP12 (F36V mutant) degron-tagged CasRx fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP12_F36V-CasRx-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker, HA-tag, and NLS
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187952-plasmid-map-sequence-id-373488

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  14. pDEST-CMV GFP-LC3B G120A

    Plasmid
    #123115
    Purpose
    Expresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.
    Depositor
    Robin Ketteler
    Publication
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Glycine 120 to Alanine
    Promoter
    CMV
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    123115-plasmid-map-sequence-id-244026

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  16. pExp-NusA

    Plasmid
    #112571
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112571-plasmid-map-sequence-id-220666

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  17. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Markus Seeger
    Publication
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Bacterial
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110095-plasmid-map-sequence-id-317064

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Showing: 6941 - 6960 of 66707 results