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pBS-PABPC

Plasmid

#26129

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-PABPC

Use

Cloning

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pBS-C3H-zfn-1

Plasmid

#26133

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-C3H-zfn-1

Use

Cloning

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pGYL1_pPromALB_FireflyLuc

Plasmid

#220282

Purpose

Firefly luciferase with minimal Albumin promoter without enhancer; enhancer of interest can be cloned into MCS

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Type

Empty backbone

Use

Luciferase

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMOD_D0000a

Plasmid

#127230

Purpose

Empty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Type

Empty backbone

Expression

Plant

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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act5c-LbCas12a

Plasmid

#140620

Purpose

Ubiquitous expression of LbCas12a in Drosophila

Depositor

Michael Boutros

Article

Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

Insert

LbCas12a

Use

CRISPR

Expression

Insect

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA

Plasmid

#21302

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

lambdaN-HA

Use

Drosophila expression

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pMOD_A5801

Plasmid

#127223

Purpose

35S::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_A5803

Plasmid

#127224

Purpose

CmYLCV::Luciferase

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSC300

Plasmid

#31820

Depositor

Yossef Av-Gay

Article

Cowley et al Gene. 2001 Feb 21;264(2):225-31.

Insert

GFP (lacking ATG start codon)

Tags

GFP (lacking ATG start codon)

Expression

Bacterial

Promoter

SOD

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK mazei

Plasmid

#108947

Purpose

heterologous expression of MVK of M. mazei in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pStA0::crtI

Plasmid

#114343

Purpose

crtI in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtI (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLEW100cre-EP1-6G

Plasmid

#33344

Depositor

George Cross

Article

Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

Insert

cre recombinase

Use

Trypanosoma brucei expression vector

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-HMGS

Plasmid

#108945

Purpose

heterologous expression of HMGS of E. faecalis in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

HMG-CoA synthase (HMGS)

Tags

His6-Tag

Expression

Bacterial

Mutation

A110G

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK jannaschii

Plasmid

#108948

Purpose

heterologous expression of MVK of M. jannaschii in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pStA0::eyfp

Plasmid

#114351

Purpose

efyp in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

efyp (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::J23116

Plasmid

#114331

Purpose

Promoter J23116 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Promoter J23116

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::RBSc42

Plasmid

#114338

Purpose

RBS RBSc42 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

RBS RBSc42

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::L3S2P55

Plasmid

#114339

Purpose

Terminator L3S2P55 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Terminator L3S2P55

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtW

Plasmid

#114347

Purpose

crtW in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtW (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtE

Plasmid

#114345

Purpose

crtE in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtE (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_proJK1_LGK

Plasmid

#81160

Purpose

Expresses levoglucosan kinase with promoter proJK1

Depositor

Timothy Whitehead

Article

Klesmith et al ACS Synth Biol. 2015 Sep 14.

Insert

Levoglucosan kinase wild-type

Tags

His6x

Expression

Bacterial

Promoter

proJK1

Available Since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-MBP_D

Plasmid

#146120

Purpose

Insect Expression of MBP

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

MBP

Expression

Insect

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDK365

Plasmid

#134195

Purpose

Retroviral plasmid with SKAP insert

Depositor

Iain Cheeseman

Insert

SKAP Alternate Start- LAP (with G5 hardeneded) (KNSTRN Human)

Use

Retroviral

Tags

LAP (GFP)

Expression

Mammalian

Mutation

Alternate Start, G5 siRNA hardened

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pStA0::crtB

Plasmid

#114344

Purpose

crtB in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtB (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::dxs

Plasmid

#114346

Purpose

dxs in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

dxs (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::crtZ

Plasmid

#114348

Purpose

crtZ in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

crtZ (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::lcyB

Plasmid

#114349

Purpose

lcyB in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

lcyB (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::idi

Plasmid

#114350

Purpose

idi in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

idi (codon optimised)

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::J23118

Plasmid

#114332

Purpose

Promoter J23118 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Promoter J23118

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pStA0::RBSc13

Plasmid

#114333

Purpose

RBS RBSc13 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

RBS RBSc13

Use

Synthetic Biology

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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