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pcDNA3.1-AntiHer2-HC197-TAG

Plasmid

#226838

Purpose

Contains trastuzumab with a TAG codon at position 197 of the heavy chain for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Bioconjug Chem. 2024 Apr 17;35(4):457-46

Inserts

anti-HER2 light chain
anti-HER2 heavy chain

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Mutation

amino acid 197 of heavy chain mutated to TAG

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-3*flag-NEXN

Plasmid

#216834

Purpose

expressing Falg tag and the coding sequence of mouse Nexn

Depositor

Yuxuan Guo

Article

Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

Insert

Nexn (mus musculus) (Nexn Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 321-843 REV1

Plasmid

#201587

Purpose

Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA repair protein REV1 (REV1 Human)

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 321-843 of DNA repair prote…

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B_aa 80-409 (delta UBL domain)

Plasmid

#201545

Purpose

Expresses a mutant form of human RAD23B that lacks the N-terminal UBL domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks N-terminal residues 1-79

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT RAD23B_delta aa 188-228 (delta UBA1 domain)

Plasmid

#201546

Purpose

Expresses a mutant form of human RAD23B that lacks the UBA1 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks residues 188-228

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_aa 1-363 RAD23B (delta UBA2 domain)

Plasmid

#201548

Purpose

Expresses a mutant form of human RAD23B that lacks the UBA2 domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Tags

FLAG

Expression

Mammalian

Mutation

Lacks C-terminal residues 364-409

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-PAM26B-F1370A-dsRNAres_L

Plasmid

#146838

Purpose

Insect Expression of DmGW182-PAM26B-F1370A-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-PAM26B-F1370A-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-delDUF-M2-dsRNAres_L

Plasmid

#146830

Purpose

Insect Expression of DmGW182-delDUF-M2-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-delDUF-M2-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-E962Q-dsRNAres_L

Plasmid

#146834

Purpose

Insect Expression of DmGW182-E962Q-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-E962Q-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-F961A-dsRNAres_L

Plasmid

#146835

Purpose

Insect Expression of DmGW182-F961A-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-F961A-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182-PAM26B-dsRNAres_L

Plasmid

#146837

Purpose

Insect Expression of DmGW182-PAM26B-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182-PAM26B-dsRNAres (gw Fly)

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEE073

Plasmid

#176830

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

712

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE074

Plasmid

#176831

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

713

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE076

Plasmid

#176833

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

715

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE078

Plasmid

#176835

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

717

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 Ub-NLRP1 S1213 P1214R

Plasmid

#166830

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Ubiquitin

Mutation

P1214R

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Centrin2

Plasmid

#136834

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin2 (CETN2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Centrin2

Plasmid

#136833

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrin2 (CETN2 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrobin

Plasmid

#136830

Purpose

Mammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrobin (CNTROB Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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gh16

Plasmid

#106700

Purpose

expression of gRNA targeting GALNT16

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT16 (GALNT16 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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