-
Plasmid#166857PurposeHuman expression vector encoding SARS CoV-2 receptor binding domain (RBD) (amino acids 319-541), containing biotinylation site and 6His tag.DepositorInsertSARS CoV-2 receptor binding domain (RBD) (S Severe acute respiratory syndrome-related coronavirus)
UseTags6xHis and Biotinylation siteExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
peSpCas9(1.1)-2×sgRNA (IFT88, donor)
Plasmid#80769PurposeExpresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).DepositorInsertIFT88 gRNA#1 (IFT88 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)
Plasmid#169189PurposeBaculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cellsDepositorInsert3xFlag-His6-nsp13 (ORF1ab SARS-CoV-2, Synthetic)
UseTags3xFlag-His6ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)
Plasmid#169180PurposeBaculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cellsDepositorInsertnsp7-His6-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
UseTagsHis6 (internal tag linking nsp7 and nsp8): nsp7-H…ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)
Plasmid#169181PurposeBaculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cellsDepositorInsertnsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
UseTagsNo purification tag and nsp7-nsp8 fusion protein …ExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ETV4_5-2)-PGKpuroBFP-W
Plasmid#211961PurposeExpress gRNA against ETV4 with puro and BFPDepositorInsertsgRNA targeting ETV4 (ETV4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)
Plasmid#169162PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cellsDepositorInsertnsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
UseTags6His-3xFlagExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)
Plasmid#169163PurposeBaculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cellsDepositorInsert3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
UseTags3xFlag-6HisExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plekhm1 -PH1/2 pGEX 4T2-GST
Plasmid#73831PurposeExpress human Plekhm1 (PH1 and PH2 domains) in bacterial cellsDepositorInsertPlekhm1 (PLEKHM1 Human)
UseTagsGstExpressionBacterialMutationto vector was cloned Ph1 and Ph2 domains of human…PromoterAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAH66 - [BAGp | gfp | tbb-2 UTR]
Plasmid#200340Purposegfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[BAGp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00001536, flp-17Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAH65 - [BAGp | wrmScarlet | tbb-2 UTR]
Plasmid#200339PurposemScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[BAGp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00001536, flp-17Available sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH71 - [AWAp | wrmScarlet | tbb-2 UTR]
Plasmid#200345PurposemScarlet BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWAp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003856, odr-10Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH72 - [AWAp | gfp | tbb-2 UTR]
Plasmid#200346Purposegfp BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWAp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00003856, odr-10Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH61 - [ASELp | wrmScarlet | tbb-2 UTR]
Plasmid#200335PurposemScarlet BioPart for ASEL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[ASELp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00001534, gcy-7Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH85 - [AWCp | wrmScarlet | tbb-2 UTR]
Plasmid#200359PurposemScarlet BioPart for AWC neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWCp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00012770, srt-47Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH51 - [DDp | wrmScarlet | tbb-2 UTR]
Plasmid#200327PurposemScarlet BioPart for DD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[DDp | wrmScarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00007304, ttr-39Available sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-UBE3A iso 2 N terminus
Plasmid#185643Purposeexpression of His-tagged UBE3A isoform 2 1-87 in bacterial cellsDepositorInsertubiquitin-protein ligase E3A (UBE3A Human)
UseTagsHisExpressionBacterialMutationnonePromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAH64 - [AFDp | gfp | tbb-2 UTR]
Plasmid#200338Purposegfp BioPart for AFD neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AFDp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00001535, gcy-8Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAH47 - [ADLp | mScarlet | tbb-2 UTR]
Plasmid#200323PurposemScarlet BioPart for ADL neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[ADLp | wrmscarlet | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00011644, T09B9.3Available sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSAH80 - [AWAp | gfp | tbb-2 UTR]
Plasmid#200354Purposegfp BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.DepositorInsert[AWAp | gfp | tbb-2 UTR]
UseTagsExpressionWormMutationPromoterWBGene00006109, str-44Available sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only