We narrowed to 1,266 results for: grna cloning vector

1. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat

   Plasmid

   #178883

   Purpose

   Vector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   U6-Nla repeat-BbsI-Nla repeat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PMXS-GOT1

   Plasmid

   #72872

   Purpose

   The retroviral GOT1 vector was generated by cloning an sgRNA resistant human GOT1 gene block into the pMXS-ires-blast vector by Gibson Assembly.

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   GOT1 (GOT1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   sgRNA resistant human GOT1, 7 silent mutations c1…

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pEE082 FoMV

   Plasmid

   #234811

   Purpose

   To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pARS3_MUbCAS9_GW

   Plasmid

   #128646

   Purpose

   T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a Gateway cassette for cloning gRNA

   Depositor

   G. Eric Schaller

   Article

   Worthen et al Development. 2019 Jun 3. pii: dev.174870

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 Puro shRNA Scramble

   Plasmid

   #162011

   Purpose

   Lentiviral negative control vector containing scramble shRNA

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   scramble shRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. sgOpti

   Plasmid

   #85681

   Purpose

   Lentiviral vector to express sgRNAs. The cloning site is BsmBI.

   Depositor

   Eric Lander, David Sabatini

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. BlatCas9

   Plasmid

   #163792

   Purpose

   The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

   Depositor

   Yongming Wang

   Article

   Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSF464

   Plasmid

   #167672

   Purpose

   Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF463

    Plasmid

    #167671

    Purpose

    Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSF278

    Plasmid

    #167673

    Purpose

    Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSF280

    Plasmid

    #167675

    Purpose

    Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSF279

    Plasmid

    #167674

    Purpose

    Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRU325

    Plasmid

    #167676

    Purpose

    Intermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pChimera

    Plasmid

    #61476

    Purpose

    Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    U6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    U6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFTK087

    Plasmid

    #171359

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    P-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN_TmiRc3_shURB5

    Plasmid

    #81066

    Purpose

    Gateway entry vector encoding shRNA to UBR5

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    shRNA targeting UBR5
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN_TmiRc3_shNT

    Plasmid

    #81067

    Purpose

    Gateway entry vector encoding non-targeting shRNA

    Depositor

    Darren Saunders

    Article

    Shearer et al Mol Biol Cell. 2018 May 9:mbcE17040248.

    Insert

    non-targeting shRNA
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Jan. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-hU6-mU6-Cre2GO

    Plasmid

    #136907

    Purpose

    Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    BlasCyGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFTK093

    Plasmid

    #171365

    Purpose

    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge