We narrowed to 974 results for: nanobody

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pAJF1124

Plasmid

#252904

Purpose

3' rpoC(MSMEG_1368)-ALFA for rpoC-ALFA in M. smegmatis via single crossover. Encodes hygromycin-resistance cassette.

Depositor

Michael Glickman

Article

Fay et al mBio. 2025 Aug 13;16(8):e0097125. doi: 1

Insert

3' rpoC(MSMEG_1368)-ALFA

Tags

ALFA tag

Expression

Bacterial

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5636

Plasmid

#250518

Purpose

Cell-free translocation reporter; translocation of a nanobody; N-terminal proOmpA-derived signal peptide with and extended hydrophobic core; C-terminal pep86 tag

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-p(extCore)OAss-nanobody-HiBiT

Tags

pep86

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pTE5629

Plasmid

#250517

Purpose

Cell-free translocation reporter; translocation of a nanobody; N-terminal YncJ signal peptide; C-terminal pep86 tag

Depositor

Tobias Erb

Article

Meier et al bioRxiv 2025.12.09.688994

Insert

T7-pYncJss-nanobody-HiBiT

Tags

pep86

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-roGFP2

Plasmid

#202462

Purpose

Expresses redox sensitive GFP (roGFP) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET-NIR-FbLAG30

Plasmid

#220748

Purpose

NIR-FbLAG30 expression in bacteria.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Expression

Bacterial

Mutation

For bacterial cytoplasmic expression, the pelB si…

Promoter

T7

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG16

Plasmid

#220749

Purpose

NIR-FbLAG16 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG16

Use

AAV and Affinity Reagent/ Antibody

Promoter

CAG

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG30

Plasmid

#220750

Purpose

NIR-FbLAG30 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

AAV and Affinity Reagent/ Antibody

Promoter

CAG

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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NbV5-TEV219S153N

Plasmid

#201488

Purpose

TANGO detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-TEV219S153N

Tags

TEV219 S153N

Expression

Mammalian

Mutation

S153N in TEV219

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-miRFP670nano3

Plasmid

#184674

Purpose

Signal anchor of rabbit cytochrome p450 and miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

CytERM-miRFP670nano3

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINO_C5

Plasmid

#171925

Purpose

Bacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Article

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_C5

Tags

His6

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pOPINO_C1

Plasmid

#171924

Purpose

Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Article

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_C1

Tags

His6

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3.0_nucLAMA-G97 IRES EGFP

Plasmid

#136620

Purpose

Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-G97-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucLAMA-F98 IRES EGFP

Plasmid

#136618

Purpose

Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-F98-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3_NSlmb-vhhGFP4

Plasmid

#35579

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Mammalian

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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SGLT1(GFP)-MAP17(nb)

Plasmid

#216210

Purpose

for expression of human GFP-tagged SGLT1-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT1 GFP fusion and MAP17-nanobody fusion (SLC5A2, PDZK1IP1 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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SGLT2(GFP)-MAP17(nb)

Plasmid

#216211

Purpose

for expression of human GFP-tagged SGLT2-MAP17 nanobody complex in BacMam system

Depositor

Lei Chen

Article

Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

Insert

SGLT2 GFP fusion and MAP17 nanobody fusion (PDZK1IP1, SLC5A2 Human)

Tags

GFP

Expression

Mammalian

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-DsbC

Plasmid

#202485

Purpose

Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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EGFR-G574V-ET1

Plasmid

#253821

Purpose

Expression of full length EGFR with G574V mutation

Depositor

Timothy Springer

Article

Watson et al J Biol Chem. 2025 Jul;301(7):110374. doi

Insert

EGFR with G574V mutation (EGFR Human)

Use

Pires2-egfp

Expression

Mammalian

Mutation

G574V

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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EGFR-R84K-ET1

Plasmid

#253818

Purpose

Expression of full length EGFR with R84K mutation

Depositor

Timothy Springer

Article

Watson et al J Biol Chem. 2025 Jul;301(7):110374. doi

Insert

EGFR with R84K mutation (EGFR Human)

Use

Pires2-egfp

Expression

Mammalian

Mutation

R84K

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

Plasmid

#199349

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOEu

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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