We narrowed to 17,014 results for: EMB

1. pSF464

   Plasmid

   #167672

   Purpose

   Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330 spCas9-mSA

   Plasmid

   #113096

   Purpose

   pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   CAS9-MSA
   

   Use

   Synthetic Biology

   Tags

   MSA (monomeric streptavidin

   Mutation

   none

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF463

   Plasmid

   #167671

   Purpose

   Intermediate vector for two gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEFIRES-P-mCherry-HPos

   Plasmid

   #87159

   Purpose

   Fluorescent marker of lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   HPos
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF-1-alpha

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRA109

   Plasmid

   #209342

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mTagBFP2 fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHIS2-TIP47 (Delta 1-190)

   Plasmid

   #17640

   Depositor

   James Hurley

   Article

   Hickenbottom et al Structure. 2004 Jul . 12(7):1199-207.

   Insert

   Tip47 (Delta 1-190) (Plin3 Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Delta 1-190

   Available Since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA5-puro-eGFP-HEC1

   Plasmid

   #114022

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mut1-RS

   Plasmid

   #182881

   Purpose

   A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

   Depositor

   Miriam Amiram

   Article

   Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

   Insert

   Mut1-RS
   

   Expression

   Bacterial

   Available Since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAV1-mEGFP

   Plasmid

   #27704

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Available Since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSC168

    Plasmid

    #216350

    Purpose

    xhNup35-Nb1t (+2Cys) Nanobody production

    Depositor

    Dirk Görlich

    Article

    Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

    Insert

    xhNup35-Nb1t (+2Cys)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    14xHis-NEDD8

    Expression

    Bacterial

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaCTag-Z13

    Plasmid

    #120056

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry
    

    Use

    Pcr template

    Tags

    mCherry

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-Akt2

    Plasmid

    #86593

    Purpose

    Expresses eGFP-tagged human Akt2(WT)

    Depositor

    Thomas Leonard, Ivan Yudushkin

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt2 (AKT2 Human)
    

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDD428

    Plasmid

    #202081

    Purpose

    Cas9/sgRNA plasmid targeting the C-terminus of Myh9

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
    

    Use

    CRISPR

    Promoter

    EF1a

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. PEP108E

    Plasmid

    #97394

    Purpose

    binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    sfGFP1-10OPT
    

    Tags

    Plasma membrane target

    Expression

    Plant

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSF-CjPglB

    Plasmid

    #199305

    Purpose

    pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    CjPglB with flag tag
    

    Tags

    Flag tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

    Plasmid

    #169097

    Purpose

    Cas9 and Citrine expression in transfected cells

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Development. 2021 Mar 9. pii: dev.193565

    Insert

    Cas9-2A-Citrine
    

    Expression

    Mammalian

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-Z07

    Plasmid

    #124790

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mNeonGreen
    

    Use

    Pcr template

    Tags

    mNeonGreen

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-flag-GFP-Intb2

    Plasmid

    #205194

    Purpose

    This vector encodes of a synCAM (intb2) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM Intb2 and GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-flag-GFP-NCAM-1

    Plasmid

    #205190

    Purpose

    This vector encodes of a synCAM (NCAM1) and GFP

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM NCAM1 and GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV Flag WT-PTEN

    Plasmid

    #22231

    Depositor

    Hong Wu

    Article

    Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

    Insert

    PTEN (PTEN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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