We narrowed to 8,531 results for: construct
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Plasmid#158033PurposeLentiviral construct with Cas9, Cre recombinase and U6 driven sgRNA cassette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)DepositorTypeEmpty backboneExpressionMammalianAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only
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pUC19/ER-mChe
Plasmid#183163PurposeConstruct for transient expression of endoplasmic reticulum-targeted mCherry in plants.DepositorInsertER-mCherry
Tags25 aa ER signal of Glycine soja kunitz-type tryps…ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hPMLIII
Plasmid#103808PurposeBLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-MFA-NFAPoptim
Plasmid#221095PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19/NLS-mChe
Plasmid#183162PurposeConstruct for transient expression of cell nucleus-targeted mCherry in plants.DepositorInsertNLS-mCherry
Tags16 aa nuclear localization signal and 16 aa nucl…ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-phiC31-LP-TC
Plasmid#183755PurposeAAVS1 targeting construct with the phiC31 landing pad cassette.DepositorInsertFRT-mCherry-attP-BleoR-F3
UseSynthetic BiologyExpressionMammalianAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFlare9A-Plekha5E10 WT
Plasmid#209576Purposeminigene construct for Plekha5 E10 alternative splicingDepositorAvailable SinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI-Myc3-HPS4
Plasmid#133931PurposeExpresses 3xMyc-HPS4 construct in mammalian cellsDepositorInsertHPS4 (HPS4 Human)
Tags3xMyc tagExpressionMammalianMutationGlutamic acid 229 to Glycine; Valine 552 to Methi…PromoterCMV I.E.Available SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBeta-lacZ-attBx2
Plasmid#124445PurposePlasmid for in vivo enhancer reporter assay. Plasmid contains a β-globin minimal promoter followed by a lacZ reporter gene derived from pRS16, with the entire reporter cassette flanked by attB sites.DepositorTypeEmpty backboneUseEnhancer reporter assay constructTagsLacZExpressionMammalianPromoterbeta globin minimal promoterAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-CYFIP1.GFP
Plasmid#109139PurposeGFP-tagged CYFIP1 expression constructDepositorAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYP1-miniSOG-citrine
Plasmid#50971PurposeAAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) constructDepositorInsertSynaptophysin-miniSOG-citrine (Syp Rat)
UseAAVTagscitrine and miniSOGPromoterhuman synapsin promoterAvailable SinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_YES1-1/1
Plasmid#91439PurposeProtein expression and purification of human SH3 domain construct YES1-1/1DepositorInsertYES1-1/1 (YES1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-Flag-BRCA1
Plasmid#71117Purposemammalian expression of Flag-BRCA1 siRNA resistant constructDepositorAvailable SinceNov. 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMVp-dsRed2-Triplex-28-gRNA1-28-pA
Plasmid#55200PurposePlasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.DepositorInsertdsRed2
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcs2-HRI-GFP-IRES-mCherry
Plasmid#226099PurposeHRI stability reporter construct for transient expression in mammalian cellsDepositorAvailable SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)
Plasmid#107939Purposepie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.DepositorInsertmCherry(dpiRNA)::ANI-1(AHPH)
ExpressionWormPromoterpie-1Available SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYP1-miniSOG-T2A-mCherry
Plasmid#50972PurposeAAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) constructDepositorHas ServiceAAV8InsertSynaptophysin-miniSOG-T2A-mCherry (Syp Rat)
UseAAVTagsT2A-mCherry and miniSOGPromoterhuman synapsin promoterAvailable SinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330_TOMM20 sgRNA / hSpCas9
Plasmid#172836PurposeMammalian expression of a sgRNA targeting the intron 4 (last intron) of TOMM20 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 4 of TOMM20 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
TRE-STOP EF1a-rtTA3
Plasmid#195882Purposecontrol construct of TRE-VEGFA EF1a-rtTA3DepositorAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only