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pCDNAHygro(+)LAMP2B

Plasmid

#86029

Purpose

Expresses LAMP2B in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Lysosome-associated membrane protein 2b (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK5 T204D

Plasmid

#80877

Purpose

mammalian expression of constitutively active ALK5 T204D

Depositor

Aristidis Moustakas

Article

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Insert

ALK5 (TGFBR1 Human)

Tags

HA

Expression

Mammalian

Mutation

T204D (constitutively active)

Promoter

CMV

Available Since

Aug. 26, 2016

Availability

Academic Institutions and Nonprofits only
Fos-FlpOERT2

Plasmid

#241239

Purpose

Expression of FlpOERT2 under the Fos promotor in mammalian cells

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

Fos-FlpoERT2

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAW240

Plasmid

#170791

Purpose

P1 integration cassette for AT110 Polymerase for integration onto Orthorep for Nanobody evolution

Depositor

Chang Liu

Article

Wellner et al Nat Chem Biol. 2021 Jun 24. pii: 10.1038

Insert

p10B2-AT110

Expression

Yeast

Promoter

10B2 (P1 specific promoter)

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)

Plasmid

#191157

Purpose

Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mTagBFP2:Bdnf.s1:mCherry

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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P55.SRE.mCMV.flag.mSrtA-3M.wpre

Plasmid

#226774

Purpose

secretory SortaseA in p53 (SRE backbone)

Depositor

Ferdinando Pucci

Article

Gonzalez-Callejo et al PLoS One. 2023 Feb 3;18(2):e0279400. doi

Insert

sortase A (srtA )

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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YY203: pAAV_cfos::mCherry-GEMINI(A)-P1N4

Plasmid

#228885

Purpose

AAV plasmid expressing the destabilized mCherry-GEMINI(A) under the control of a cfos promotor.

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

mCherry-GEMINI(A)

Use

AAV

Tags

mCHERRY

Expression

Mammalian

Promoter

cfos

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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YY225: pAAV_TRE::GFP- GEMINI(A)-P1N4

Plasmid

#228888

Purpose

AAV plasmid expressing the destabilized GFP-GEMINI(A) under the control of a doxycycline-inducible promotor.

Depositor

Dingchang Lin

Article

Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-

Insert

GFP-GEMINI(A)

Use

AAV

Tags

gfp

Promoter

TRE

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pRosetta_v2

Plasmid

#136477

Purpose

Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

Depositor

John Doench

Article

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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