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pGEX-mTAZ-WW (43)

Plasmid

#40916

Depositor

Michael Yaffe

Article

Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Insert

TAZ (Wwtr1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Contains mTAZ WW domain (43 amino acids, 122-164)

Promoter

Tac

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
psbA2-Ptrc-T7-g10-PHLS (e)

Plasmid

#52310

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc-T7

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-Dnm2b-DN

Plasmid

#109574

Purpose

Multisite gateway vector for 3' tagging with dominant negative Dynamin2b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2b (dnm2b Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf175

Plasmid

#12863

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf175

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
pMT1100

Plasmid

#8618

Depositor

Robert Hartley

Article

Hartley et al Protein Peptide Letters 1996 3:225-231

Insert

ribonuclease Sa3, barstar

Tags

phoA signal sequence to RNase Sa3

Expression

Bacterial

Mutation

In addition to the substitution of the barnase ge…

Available Since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only
pYNL-N1_zyxin

Plasmid

#65681

Purpose

Expresses Zyxin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf256

Plasmid

#12944

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf256

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pCNL-N1_3xNLS

Plasmid

#65673

Purpose

Expresses NLS-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_3xNLS

Plasmid

#65672

Purpose

Expresses NLS-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf234

Plasmid

#12922

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf234

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pSC-hXRCC1-PreE2

Plasmid

#52278

Purpose

Bacterial expression of human XRCC1 C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

XRCC1 (XRCC1 Human)

Tags

Ubc9-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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psbA2-PHLS (b)

Plasmid

#52307

Purpose

Replaces the psbA2 gene in Synechocystis with the PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

psbA2

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf226

Plasmid

#12914

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf226

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
psbA2-Ptrc-PHLS (d)

Plasmid

#52309

Purpose

Replaces the psbA2 gene in Synechocystis with the Ptrc promoter - PHLS gene

Depositor

Anastasios Melis

Article

Formighieri et al Planta. 2014 Aug;240(2):309-24. doi: 10.

Inserts

beta-phellandrene synthase
Chloramphenicol resistance

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptrc

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir129-2-wt

Plasmid

#46730

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir129-2-wt (MIR129-2 Synthetic, Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPR sgSHMT2_2

Plasmid

#106310

Purpose

Express Cas9 and sgRNA targeting SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAB085d7

Plasmid

#79211

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnCAD-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAB085d6

Plasmid

#79210

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PFA0445w-bio-His

Plasmid

#50811

Purpose

Expresses enzymatically monobiotinylated full length PFA0445w

Depositor

Gavin Wright

Article

Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

Insert

PFA0445w

Tags

ratCD4d3+4,biotinylation sequence, 6xHis

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Feb. 13, 2014

Availability

Academic Institutions and Nonprofits only

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