We narrowed to 16,357 results for: Jun
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Plasmid#83489Purposecre-dependent PAK1-AID under EF1a promoterDepositorInsertPAK1-AID-ires-EGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(T17N)
Plasmid#164902PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domainDepositorInsertRaichuEV-Rac1 (RAC1 Human)
UseTagsExpressionMammalianMutationT17N mutation in the Rac1 domain which make the s…PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-7-5p
Plasmid#103723PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-7-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-7-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA
Plasmid#154776Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation102TAG 150TAA in GFP reporter, hybrid PylT with A…PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330_ACTB-i4 sgRNA / hSpCas9
Plasmid#172828PurposeMammalian expression of a sgRNA targeting the intron 1 position 4 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNAIIIB HA JNK
Plasmid#61630Purposemammalian expression of JNKDepositorInsertJNK1 beta1 isoform (MAPK8 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry
Plasmid#83490Purposecre-dependent CA-AMPK under EF1a promoterDepositorInsertCA-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationH150RPromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT5T_hp53_ST
Plasmid#34922DepositorInsertp53 DNA-binding (Res 1-393) (TP53 Human)
UseTagsExpressionBacterialMutationC135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …PromoterT7Available sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Actin-7
Plasmid#58001PurposeLocalization: Actin, Excitation: 555, Emission: 584DepositorInsertActin (ACTB Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEMHE-NLS-Beta-catenin-S33Y
Plasmid#138537PurposeFor making Beta-catenin-S33Y RNADepositorInsertBeta-catenin
UseIn vitro transcriptionTags2XFLAG and 2xNLSExpressionMutationchanged serine 33 to tyrosinePromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Cre
Plasmid#47117PurposeCan be used to in vitro transcribe Cre recombinase.DepositorInsertCre
UseTagsExpressionMutationPromoterLacAvailable sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTBL437 AAVS1 EQR sgRNA
Plasmid#126447PurposeTo cut the human AAVS1 locus for integration.DepositorInsertspacer against human AAVS1 safe-harbor locus with NGAG PAM
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 150TAG
Plasmid#154772Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation150TAG in GFP reporter, hybrid PylT with A41AA an…PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-101-3p
Plasmid#103165PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-Golgi-7
Plasmid#57959PurposeLocalization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565DepositorInsertGolgi (B4GALT1 Human)
UseTagsmOrange2ExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMX-SHP1C-ires-hCD8
Plasmid#183720PurposeExpresses the myc-tagged catalytic domains of wild-type SHP-1.DepositorInsertSHP-1
UseRetroviralTagsExpressionMutationcatalitic domain alonePromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD9 Flag
Plasmid#74901PurposeExpress COMMD9 in mammalian cellsDepositorInsertCOMMD9 (COMMD9 Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
VVT1
Plasmid#65779PurposeThe Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNADepositorInsertSaCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SHP2 D61A
Plasmid#8330DepositorInsertSHP2 (PTPN11 Human)
UseRetroviralTagsExpressionMammalianMutationD61A (activated mutant)PromoterAvailable sinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
inhibin-DsRed
Plasmid#156184PurposeExpression plasmid for production of transgenic rainbow trout strain carrying the DsRed gene under control of the inhibin α promoter.DepositorInsertsrainbow trout inhibin α promoter
rainbow trout β actin 3'UTR
UseTagsExpressionBacterialMutationPromoterinhibinAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only