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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,430 results for: BLI

Showing: 7021 - 7040 of 8430 results

  1. pFP.R358

    Plasmid
    #138228
    Purpose
    Expresses the split mCherry reporter interrupted by the CIV-RIR1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).CIV-RIR1*.(CSE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138228-plasmid-map-sequence-id-273508

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  2. pFP.R359

    Plasmid
    #138229
    Purpose
    Expresses the split mCherry reporter interrupted by the CP-Thy1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(KHH).CP-Thy1*.(SVL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138229-plasmid-map-sequence-id-273505

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  3. pFP.R361

    Plasmid
    #138231
    Purpose
    Expresses the split mCherry reporter interrupted by the BsuP-RIR1 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).BsuP-RIR1*.(CSE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138231-plasmid-map-sequence-id-273489

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  8. pTEI127

    Plasmid
    #126067
    Purpose
    Module 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Insert
    mitochondria Rieske iron sulphur protein (1-100 aa)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Mutation
    Promoter
    'long' CaMV35S
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126067-plasmid-map-sequence-id-246127

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  9. pTEI177

    Plasmid
    #126077
    Purpose
    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    ccdB Cassette (BsaI)
    GFP1-10
    Use
    Binary, module 1
    Tags
    linker (GTGGGGS)
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126077-plasmid-map-sequence-id-246860

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  10. pTEI129

    Plasmid
    #126210
    Purpose
    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    ccdB Cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary, module 1
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126210-plasmid-map-sequence-id-246910

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  12. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

    Plasmid
    #117140
    Purpose
    Express gRNA vs Dmap1
    Depositor
    Kosuke Yusa
    Insert
    gDmap1 v1 (Dmap1 Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117140-plasmid-map-sequence-id-233768

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  13. pTH825-CEN-minRLuc/slowstaCFLuc

    Plasmid
    #115370
    Purpose
    Expresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferase
    Depositor
    Tobias von der Haar
    Inserts
    Gcn4 5'-UTR + Firefly Luciferase
    Renilla luciferase (codon minimised)
    Use
    Tags
    Expression
    Yeast
    Mutation
    All codons have been changed for the least favour…
    Promoter
    ADH1 and TDH3
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    115370-plasmid-map-sequence-id-238823

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  14. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

    Plasmid
    #107301
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
    Use
    CRISPR
    Tags
    FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107301-plasmid-map-sequence-id-204785

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  15. pQE80L TriCatcher-MMP

    Plasmid
    #112632
    Purpose
    Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher
    Depositor
    Mark Howarth
    Article
    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:
    Insert
    TriCatcher-MMP
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    central matrix metalloproteinase-cleavable sequen…
    Promoter
    T5
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112632-plasmid-map-sequence-id-221417

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  16. pBABE.puro/TO/Flag/HuR.S221D

    Plasmid
    #110347
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG tag
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    S221D, P237S
    Promoter
    gag
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110347-plasmid-map-sequence-id-218849

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  17. pBABE.puro/TO/Flag/HuR.S221D

    Plasmid
    #110355
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and mKO2 tags
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG and mKO2
    Expression
    Mammalian
    Mutation
    S221D, P237S
    Promoter
    gag
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110355-plasmid-map-sequence-id-213699

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  18. pcDNA3.1 Flag YFP hNKCC1 A720C C723S C724V (NT534)

    Plasmid
    #50899
    Purpose
    Expresses human NKCC1 A720C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A720C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50899-plasmid-map-sequence-id-76372

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  19. pcDNA3.1 Flag YFP hNKCC1 L718C C723S C724V (NT532)

    Plasmid
    #50897
    Purpose
    Expresses human NKCC1 L718C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L718C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50897-plasmid-map-sequence-id-76370

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  20. pcDNA3.1 Flag YFP hNKCC1 L722C C723S C724V (NT536)

    Plasmid
    #50901
    Purpose
    Expresses human NKCC1 L722C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L722C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50901-plasmid-map-sequence-id-76374

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Showing: 7021 - 7040 of 8430 results