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pET28a-UVRAG CC

Plasmid

#24400

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

UVRAG (UVRAG Human)

Tags

His

Expression

Bacterial

Mutation

Contains on the CC domain, amino acids 200-270

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac-His-Tipin

Plasmid

#22891

Depositor

Aziz Sancar

Article

Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Insect

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1 shMTH1-1

Plasmid

#21297

Depositor

Bob Weinberg

Article

Rai et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

shMTH1-1 (NUDT1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
p406MET3

Plasmid

#17436

Depositor

Nicolas Buchler, Fred Cross

Insert

MET3 promoter + polylinker + CYC1 terminator (MET3 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

MET3 promoter is between SacI and XbaI restrictio…

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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pACS145

Plasmid

#204600

Purpose

Histone H3.3K36M-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

histone H3.3 K36M (H3-3A Human)

Use

Lentiviral

Tags

human histone H3.3 K36M - V5

Expression

Mammalian

Mutation

histone H3.3 K36M

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMCB306-GFP-Rab29

Plasmid

#198472

Purpose

lentiviral expression of GFP-Rab29

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

GFP-Rab29 (RAB29 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1A

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:mNeonGreen-Rab5a

Plasmid

#192355

Purpose

zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

Rab5a (rab5aa Zebrafish)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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AiP995-pAAV-mscRE10-minBGpromoter-EGFP-WPRE-hGHpA

Plasmid

#163485

Purpose

Direct-expressing EGFP AAV Virus. Alias: AiP995 - pAAV-AiE2010m-minBG-EGFP-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

EGFP

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AiP1011-pAAV-mscRE4-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163480

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1011 - pAAV-AiE2004m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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KX002: pMVP/PB-DEST

Plasmid

#121869

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_Sik3

Plasmid

#119972

Purpose

Lentiviral expression plasmid of mouse Sik3 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Sik3 (Sik3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EFS promoter

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP305-pAAV-EFS-dSaCas9-KRAB-pA

Plasmid

#113681

Purpose

A EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

de-catalyzed SaCas9

Use

AAV and CRISPR

Tags

KRAB and NLS

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Plxnb1(L)-Fc-His

Plasmid

#72126

Purpose

Expresses the entire extracellular region of the PlexinB1 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb1 (Plxnb1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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ROZA-XL

Plasmid

#64194

Purpose

Fluorescent reporter for ZAP-70 tyrosine kinase activity

Depositor

Annemarie Lellouch

Article

Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

Insert

ROZA-XL

Expression

Mammalian

Mutation

ROZA-XL

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pSC-IntE2

Plasmid

#52265

Purpose

Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

Ubc9 GST fusion (UBE2I Human, Synthetic)

Tags

GST and GyrA-intein

Expression

Bacterial

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pSIL-eGFP-shACTN1

Plasmid

#52676

Purpose

shRNA against α-actinin-1 with eGFP transfection marker

Depositor

Johannes Hell

Article

Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

Insert

ACTN1 (Actn1 Rat)

Use

RNAi

Tags

CMV-EGFP

Promoter

U6

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCru5-/GCCACC-mEGFP-IRES-mCherry

Plasmid

#49226

Purpose

Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

Depositor

Clifford Wang

Article

Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

Inserts

mEGFP
mCherry

Use

Retroviral and Synthetic Biology

Tags

SfuI site to create C-terminal fusions

Expression

Mammalian

Promoter

Viral LTR (or CMV)

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)

Plasmid

#48287

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and msfGFP

Expression

Bacterial

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pIE2-AD

Plasmid

#21603

Depositor

Takahiro Kusakabe

Article

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

NF-kappa-B p65 subunit (Rela Mouse)

Use

Gateway Cloning

Tags

Flag, Gateway destination cassette, and NLS

Expression

Insect

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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gTBEv3

Plasmid

#220619

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv3) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

Insert

hUNG2-nSpCas9

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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