We narrowed to 9,521 results for: try

1. pME iCre-I (JDW 1203)

   Plasmid

   #224520

   Purpose

   A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   iCre-I
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3E-H2A_mCherry_SV40pA (JDW 967)

   Plasmid

   #224528

   Purpose

   A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2A-mCherry-SV40pA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

   Plasmid

   #224532

   Purpose

   A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-H2A-mCherry-SV40pA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E EFS-rtTA/rtTA3 (JDW 1214)

   Plasmid

   #224533

   Purpose

   A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   rtTA
   

   Use

   Gateway cloning

   Promoter

   EFS

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

   Plasmid

   #224534

   Purpose

   A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh-mNeonGreen-HA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-IRES-mTagBFP2-pA (JDW 1361)

   Plasmid

   #224535

   Purpose

   A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   IRES-FLAG-NLS-mTagBFP2-pA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

   Plasmid

   #224538

   Purpose

   A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Lifeact-mScarlet-HA
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME H2B mCerulean (JDW 1150)

   Plasmid

   #224543

   Purpose

   A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   H2B-mCerulean
   

   Use

   Gateway cloning

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E 5X C120 c-fos min pro (JDW 1241)

   Plasmid

   #224544

   Purpose

   A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   5x-C120-c-Fos promoter
   

   Use

   Gateway cloning

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221-eGFP_myr

    Plasmid

    #221412

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    eGFP_myr
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-eGFP

    Plasmid

    #221413

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    eGFP
    

    Expression

    Mammalian

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HTR1E-DuET

    Plasmid

    #213319

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    HTR1E (HTR1E Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Qt-FLAG-TGA-rt9us-antiGFP

    Plasmid

    #206954

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Expression

    Mammalian

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR4 CDS A. thaliana MAMI

    Plasmid

    #209186

    Purpose

    pENTR4 plasmid with CDS of Arabidopsis thaliana MEMBRANE-ASSOCIATED MANNITOL-INDUCED (MAMI, AT5G54110.1) including stop codon for N-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    AT5G54110.1 (MAMI Mustard Weed)
    

    Use

    Gateway-compatible entry vector

    Promoter

    none

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFSynW SYT9 D197, 199N IRES GFP

    Plasmid

    #195701

    Purpose

    Lentiviral plasmid encoding SYT9 with D197, 199N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Mutation

    full-length mouse SYT9 with D197, 199N mutations

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFSynW SYT9 D330, 332N IRES GFP

    Plasmid

    #195702

    Purpose

    Lentiviral plasmid encoding SYT9 with D330, 332N mutations followed by an internal ribosomal entry site followed by EGFP under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Syt9 (Syt5 Mouse)
    

    Use

    Lentiviral

    Mutation

    full-length mouse SYT9 with D330, 332N mutations

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. Qt-FLAG-TAA-rt20s-antiGFP

    Plasmid

    #206955

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1M-Qt-FLAG-TGA-rt20s-antiGFP

    Plasmid

    #206953

    Purpose

    Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, QtEnc, antiGFP-intrabody
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. eDrMet

    Plasmid

    #183999

    Purpose

    Optically controlled eDrcMet expression in mammalian cells

    Depositor

    Vladislav Verkhusha, Dmitry Gerashchenko

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrMet (MET Human)
    

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. KTK_113

    Plasmid

    #180540

    Purpose

    Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Insert

    Signal peptide region of CsgA including SecB-N22 sequenced
    

    Expression

    Bacterial

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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