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pCAG/ATP11C-c-HA

Plasmid

#209222

Purpose

Mammalian expression of ATP11C isoform c

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform c (ATP11C Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11C-b-HA

Plasmid

#209221

Purpose

Mammalian expression of ATP11C isoform b

Depositor

Hye-Won Shin

Article

Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

Insert

ATP11C isoform b (ATP11C Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP10A(E203Q)-HA

Plasmid

#209258

Purpose

Mammalian expression of ATP10A mutant E203Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10A (ATP10A Human)

Use

Retroviral

Tags

HA

Mutation

E203Q

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10A(E203Q)-HA

Plasmid

#209246

Purpose

Mammalian expression of ATP10A mutant E203Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10A (ATP10A Human)

Tags

HA

Expression

Mammalian

Mutation

E203Q

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 BBS exon37a pCAGGS

Plasmid

#206086

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and alternative exon 37a

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-RnCENP-O-GFP

Plasmid

#205179

Purpose

Expresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

Depositor

Michael Lampson

Article

Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

Insert

CENP-O (Cenpo Rat)

Use

In vitro transcription

Tags

5 glycines linker followed by GFP

Expression

Mammalian

Mutation

Wild type rat CENP-O

Promoter

T7

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW pHluorin-SYT9 IRES mRuby3

Plasmid

#195698

Purpose

Lentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Rat)

Use

Lentiviral

Tags

pHluorin

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynW Syt9 IRES mRuby3

Plasmid

#195700

Purpose

Lentiviral plasmid encoding full-length mouse SYT9 followed by an internal ribosomal entry site followed by mRuby under the human synapsin promoter

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Use

Lentiviral

Mutation

encodes only the first 68 amino acids of Tac1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2B D330, 332N

Plasmid

#195705

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2B domain with D330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 253-344 of mouse SYT9 with D3…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2AB D197,199,330,332N

Plasmid

#195706

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2AB domain with D197, 199, 330, 332N mutations

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 104-386 of mouse SYT9 with D1…

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pHD22Y/PfPRMT5 deletion

Plasmid

#203161

Purpose

for disruption of PfPRMT5 gene

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment

Tags

3 myc tags and 3' UTR of P. berghei dihydrof…

Expression

Mammalian

Promoter

No

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-flag-Lag16-fibcon-iCAM1-tether

Plasmid

#205213

Purpose

This vector encodes of the synCAM tether (fibcon - iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM fibcon - iCAM1 Tether and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW1-GlcisPT

Plasmid

#203172

Purpose

Yeast expression vector for Giardia cis-prenyltransferase

Depositor

William Sessa

Article

Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

Insert

UPPS

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pASPIre4

Plasmid

#196656

Purpose

Derivative of pASPIre3. Contains SpeI restriction site within the CDS of bxb1 to enable diversification of the 5’-UTR and codons 2-16.

Depositor

Markus Jeschek

Article

Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

Insert

Bxb1-sfGFP fusion controlled by rhamnose promoter; attB/attP-flanked discriminator; exchangable 5'-UTR and CDS (codons 1-16)

Expression

Bacterial

Mutation

SpeI site in Bxb1 CDS

Promoter

rhamnose-inducible promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWalium Htt19Q-mCherry

Plasmid

#201248

Purpose

allows the expression of the insert in Drosophila melanogaster

Depositor

Pedro Domingos

Article

Branco-Santos et al Hum Mol Genet. 2017 Oct 1;26(19):3763-37

Insert

Huntingtin (HTT Human)

Expression

Insect

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo E321K ORF29

Plasmid

#200025

Purpose

Lentiviral vector for expression of KSHV ORF29 with E321K mutation

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Mutation

Glutamic acid 321 changed to lysine

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo G226A E321K ORF29

Plasmid

#200026

Purpose

Lentiviral vector for expression of KSHV ORF29 with G226A and E321K mutation

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Mutation

Glycine 226 changed to alanine, glutamic acid 321…

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo D476A ORF29

Plasmid

#200027

Purpose

Lentiviral vector for expression of KSHV ORF29 with D476A mutation

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Mutation

Aspartic acid 476 changed to Alanine

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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Control V5-Twist

Plasmid

#201376

Purpose

Expresses Twist for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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MT2A_beta-Villin

Plasmid

#200301

Purpose

Bacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

beta-domain of metallothionein 2a in fusion with HP-35 (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Promoter

T7

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D]-FLAG

Plasmid

#199315

Purpose

mutated phosphorylation site (kinase acitivity permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Aspartic Acid

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199A]-FLAG

Plasmid

#199313

Purpose

mutated phosphorylation site (kinase acitivity inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-basic

Plasmid

#196882

Purpose

Used for preparation of double-floxed inverse ORF vectors

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Type

Empty backbone

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BBLF2/3

Plasmid

#195831

Purpose

Gateway Entry Clone of Epstein-Barr virus BBLF2/3

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BBLF2/3 (BBLF2/BBLF3 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BcRF1(vTBP)

Plasmid

#195832

Purpose

Gateway Entry Clone of Epstein-Barr virus BcRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BcRF1 (BcRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BCRF1(vIL10)

Plasmid

#195777

Purpose

Gateway Entry Clone of Epstein-Barr virus BCRF1, vIL-10

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BCRF1, vIL-10 (BCRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BaRF1(RNR-s)

Plasmid

#195795

Purpose

Gateway Entry Clone of Epstein-Barr virus BaRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BaRF1 (BaRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-CI

Plasmid

#196852

Purpose

Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (R354H)

Plasmid

#197010

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

GFP

Expression

Mammalian

Mutation

R354H

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/TO-GFP-SPOP (R221C)

Plasmid

#197009

Purpose

Mammalian inducible expression vector for GFP-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

GFP

Expression

Mammalian

Mutation

R221C

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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