We narrowed to 30,217 results for: PLE;

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pMCP-RecT

Plasmid

#183827

Purpose

pLenti-EF1A-MCP-EXTEN-RecT-NLS

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

MCP-RecT_NLS

Expression

Mammalian

Promoter

EF1A

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCAT001

Plasmid

#171061

Purpose

Plasmid for expressing HIV-1 envelope glycoprotein

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

HIV-1 Env

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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HA-cdc20

Plasmid

#11594

Depositor

Marc Kirschner

Article

Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

Insert

cdc20 (CDC20 Human)

Tags

HA

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

Plasmid

#175544

Purpose

Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag9-myc_PDGFRtmb

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCR-U6-gRNA-TRE3G-TdTomato

Plasmid

#192658

Purpose

A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

TdTomato

Expression

Mammalian

Promoter

U6, TRE3G

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

Plasmid

#50470

Purpose

Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter

Depositor

Bryan Roth

Insert

hM3D(Gq)-IRES-mCitrine (CHRM3 Human)

Use

AAV

Tags

HA

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

Plasmid

#225756

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-puro-mIR-31

Plasmid

#26088

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

miR-31 (MIR31 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 3, 2010

Availability

Academic Institutions and Nonprofits only
pENTR1A-GFP-N2 (FR1)

Plasmid

#19364

Purpose

Gateway entry vector for C-term eGFP fusion, frame N2

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced Green Fluorescent Protein

Use

Gateway entry vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Tags

6xHIS and MBP

Expression

Bacterial

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
pCAG ERT2-Cre-ERT2 lox

Plasmid

#183447

Purpose

CreOFF tamoxifen-inducible Cre

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

ERT2-Cre-ERT2

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-iRFP702-LaminB1

Plasmid

#184456

Purpose

Nuclear membrane-targeted iRFP702 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Article

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Insert

iRFP702-LaminB1

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP200-Lenti-TRE3G-Flag-GluN2A-WPRE-pA

Plasmid

#164724

Purpose

pLenti vector backbone designed to express Flag-GluN2A from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2A

Use

Lentiviral

Tags

Flag

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA

Plasmid

#62930

Purpose

pLenti vector backbone designed to express GFP from a 1.1Synapsin promoter .

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Promoter

1.1Synapsin and 1.3αCaMKII

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huMb2Cas12a

Plasmid

#174676

Purpose

Expresses 6xHis-MBP tagged Mb2Cas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huMb2Cas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV and NLS, 6xHis, 3xHA

Expression

Bacterial

Promoter

T7

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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mTOR_1 shRNA

Plasmid

#1855

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

mTOR (MTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

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pCAG-NL1(-)

Plasmid

#15260

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 1 (Nlgn1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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FLAG LOC550643 pFCPGW

Plasmid

#86853

Purpose

retroviral expression of NoBody

Depositor

Sarah Slavoff

Article

D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

Insert

LOC550643 (NBDY )

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMOS011: superecliptic pHluorin (mitochondrial)

Plasmid

#163050

Purpose

superecliptic pHluorin (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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XBP-1 2: pFLAG.XBP1p.CMV2

Plasmid

#21833

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 processed/spliced (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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