We narrowed to 7,234 results for: CAR
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Plasmid#179844Purposedonor plasmid for cardiac-specific CCND2-T2A-luciferase expression in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR, Luciferase, and TALEN ; Donor plasmidTagsluciferaseExpressionMammalianPromoterTroponin T promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_W208L
Plasmid#221486PurposeNKX2-1 W208L DNA binding mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationW208LPromoterEF1aAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX-EF1a_NKX2-1_V205F
Plasmid#221485PurposeNKX2-1 V205F DNA binding mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationV205FPromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TNT4-TadA8e-SpRY N aa2-713-InteinN-miR122 TS
Plasmid#209787PurposeExpresses TadA8e and SpRY cas9N by the specific TNT4 promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)DepositorInsertTNT4, TadA8e, SpRY N, inteinN, miR122 TS
UseAAVPromoterTNT4Available sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2M-SDMutation-EhT
Plasmid#215546PurposeDonor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-outDepositorInsertB2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
UseCRISPRMutationThe second base of the first intron of B2M (From …Available sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Creb5 shRNA
Plasmid#195020PurposeBovine Creb5 shRNA targeting the Creb5 3′UTRDepositorInsertCreb5 shRNA (CREB5 Bovine)
ExpressionMammalianAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AtSLAC1 WT
Plasmid#212946PurposeVector used for expression of AtSLAC1 WT in mammalian cellDepositorAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Alpha-2C adrenergic receptor [N330A/80R]
Plasmid#206555PurposeMammalian Expression Plasmid of anti-Alpha-2C adrenergic receptor (Human). Derived from hybridoma N330A/80.DepositorInsertanti-Alpha-2C adrenergic receptor (Homo sapiens) recombinant Mouse monoclonal antibody (ADRA2C Mouse)
ExpressionMammalianPromoterDual CMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NLS-YFP-CCTc
Plasmid#184327PurposeMammalian expression of the nuclear localized YFP-CCTd(peptide of Cav1.2)DepositorInsertCCTc (CACNA1C Human)
ExpressionMammalianAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 1
Plasmid#175814PurposepENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 1 (AT3G06720) without stop codon for C-terminal epitope tagsDepositorInsertIMPORTIN ALPHA ISOFORM 1 (IMPA-1 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnonePromoternoneAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_d1_Ub
Plasmid#170729PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
Tags6xHis-TEVExpressionBacterialMutationThe C-terminus of OTC was truncated by removing o…PromoterT7Available sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-405(del30)
Plasmid#166128PurposeExpression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertT1head1-405(del30) (TLN1 Human)
TagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405, with 30 amino acid deletion in th…Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
t1-405(T144E,T150E)
Plasmid#166130PurposeFor expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertHuT1head1-405(T144E,T150E) (TLN1 Human)
TagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-405, mutations T144E and T150EAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)
Plasmid#166855PurposeExpresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeastDepositorInsertAtg13[571–700] (ATG13 Budding Yeast)
Tags6xHisExpressionYeastMutationK683E, H685E, K686EPromoterpCuAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R
Plasmid#166810PurposeMammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association.DepositorAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2ABFP-W
Plasmid#163170PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tagDepositorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W
Plasmid#163172PurposeLentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tagDepositorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK-Ptrf-mKate2
Plasmid#128765PurposeFluorescent mKate reporter for PtrfDepositorAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Myosin7B-sgRNA
Plasmid#154861PurposeLentiviral expression of Cas9 and sgRNA targeting Myosin7BDepositorAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W
Plasmid#105028PurposeLentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFPDepositorAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only