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pLentiCRISPR V2 Neo sgITGA5_1

Plasmid

#233251

Purpose

Knock out of murine ITGA5

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

ITGA5 (Itga5 Mouse)

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 Neo sgAGAP1_3

Plasmid

#233248

Purpose

Knock out of murine AGAP1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

AGAP1 (Agap1 Mouse)

Use

Lentiviral

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 Neo PTEN sgRNA2

Plasmid

#233245

Purpose

Knock out of murine PTEN

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

PTEN (Pten Mouse)

Use

Lentiviral

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 Neo sgAGAP1_2

Plasmid

#233247

Purpose

Knock out of murine AGAP1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

AGAP1 (Agap1 Mouse)

Use

Lentiviral

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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MKC176_EPOR(2A-synEPOR)

Plasmid

#232414

Purpose

AAV production plasmid for EPOR(synEPOR) vector from Figs. 3-5 that mediates HDR at EPOR locus using EPOR gRNA. synEPOR is codon diverged to prevent premature recombination with EPOR locus.

Depositor

Kyle Cromer

Article

Shah et al Nat Commun. 2025 Jan 29;16(1):1140. doi:

Insert

Truncated Erythropoietin Receptor (EPOR Human)

Use

AAV

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

Plasmid

#223323

Purpose

Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Insert

SpCas9 and sgRNA-T2 (cas9 )

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

U6

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_ZIKV_NS5_MTase

Plasmid

#213529

Purpose

Expresses the MTase domain of NS5 of ZIKV

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

ZIKA virus NS5 methyltransferase domain

Tags

His6-sumo

Expression

Bacterial

Mutation

MTase domain aa 1-265

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

Plasmid

#220609

Purpose

Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory hM4D(Gi) DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

FLAG-hM4D(Gi)-P2A-ArgiNLS-AausFP1

Use

AAV and Cre/Lox

Tags

FLAG; ArgiNLS

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-EWS_RRM-RGG(359-513)

Plasmid

#188046

Purpose

Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Biopolymers. 2023 May;114(5):e23536. doi

Insert

EWSR1 (EWSR1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-358, 514-656

Promoter

T7

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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macroH2A1.2-F192V-GFP (pc5115)

Plasmid

#223438

Purpose

Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.2-F192V (Macroh2a1 Rat)

Tags

GFP

Expression

Mammalian

Mutation

Mutation of phenylalanine 192 to valine (F192V)

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-Anillin deltaAnillin homology domain

Plasmid

#187277

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Anillin homology domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Mutation

deltaAnillin homology domain

Promoter

U6, CMV

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dFnCas9

Plasmid

#201954

Purpose

Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dFnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A and H969A on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en1dFnCas9GFP

Plasmid

#201950

Purpose

Expression of en1dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en1dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1369R on FnCas9

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en15dFnCas9GFP

Plasmid

#201951

Purpose

Expression of en15dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en15dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1603H on FnCas9

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dSpCas9

Plasmid

#201953

Purpose

Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dSpCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A on SpCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgFAAH

Plasmid

#209197

Purpose

Mutagenesis of Faah

Depositor

Larry Zweifel

Article

Balsevich et al Elife. 2023 Apr 11;12:e81919. doi: 10.75

Insert

Faah (Faah Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Promoter

CMV

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE FL NAP1 STOP C' EGFP IRES Blast

Plasmid

#179102

Purpose

Expresses untagged full length NAP1

Depositor

Alicia Pickrell

Article

Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

Insert

Nak associated protein 1 (AZI2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-N'-FLAG-HA-NAP1S318A

Plasmid

#195410

Purpose

Expresses FLAG-HA tagged human NAP1 with S318A point mutation

Depositor

Alicia Pickrell

Article

Paul et al J Cell Biol. 2024 Feb 5;223(2):e20230308

Insert

Nak associated protein 1 (AZI2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Serine 318 turned to Alanine

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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ERAP1-rs27895(T)

Plasmid

#206143

Purpose

Expresses the rs27895-T allele of ERAP1, myc-tagged.

Depositor

Dennis Ko

Article

Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

Insert

ERAP1 (ERAP1 Human)

Tags

myc

Mutation

G346 changed to D346

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.3

Plasmid

#206137

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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