We narrowed to 7,572 results for: Lif

1. pCS2.0 Tag-RFP-T-UtrCH

   Plasmid

   #101279

   Purpose

   Life imaging pCS2.0 Tag-RFP-T_UtrCH

   Depositor

   Alpha Yap

   Article

   Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

   Insert

   Utrophin (UTRN Human)
   

   Use

   Tags

   TagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST47-ARL1-GFP

   Plasmid

   #67395

   Purpose

   Mammalian expression of hArl1 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL1 (ARL1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   bp 231 C to T; silent mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDONR P2R-P3-EGFP

   Plasmid

   #48349

   Purpose

   Contributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Aminoacid 40 is valine in our sequence, while it …

   Promoter

   none

   Available since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST47-ARL8B

   Plasmid

   #67467

   Purpose

   Mammalian expression of hArl8b with stop codon (native expression)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8B (ARL8B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET-DEST42-ARF6-V5-His6

   Plasmid

   #67415

   Purpose

   Expression of hArf6 with C-term V5 and His6 tag in E. coli

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Tags

   His and V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7/lacO

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

   Plasmid

   #119067

   Purpose

   Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST47-ARL6-GFP

   Plasmid

   #67401

   Purpose

   Mammalian expression of hArl6 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL6 (ARL6 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pDONR221-ARL8B

   Plasmid

   #67334

   Purpose

   hArl8b with stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8B (ARL8B Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

   Plasmid

   #119065

   Purpose

   Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

   Depositor

   Tatjana Sauka-Spengler

   Article

   Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

   Insert

   HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-CYLD-C601A

    Plasmid

    #60028

    Purpose

    N-terminal GFP tagged catalytic inactive (C601A) expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Cysteine 601 changed to Alanine

    Promoter

    CMV

    Available since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR P2R-P3-V5-6xHis

    Plasmid

    #48347

    Purpose

    Contributes the coding sequence of a V5-6xHis epitope tags cassette as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    V5 and 6xHis epitope tags cassette
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Contains a stop codon at the end of the epitope t…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-Tight-FLAG-CYLD-C601A

    Plasmid

    #60029

    Purpose

    CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cysteine 601 changed to Alanine

    Promoter

    TRE

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pR2Q347y

    Plasmid

    #112680

    Purpose

    functional, fluorescent, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Use

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    cmV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-TAF15N-miRFP670-Cry2WT

    Plasmid

    #122440

    Purpose

    Expresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    TAF15 (TAF15 Human)
    

    Use

    Lentiviral

    Tags

    Cry2WT and miRFP670

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-208

    Promoter

    SFFV

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST40-ARF6-V5-His6

    Plasmid

    #67436

    Purpose

    Mammalian expression of hArf6 with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF6 (ARF6 Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.2-Cx43-siResist

    Plasmid

    #49852

    Purpose

    Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains several wobble mutations to confer resis…

    Promoter

    CMV

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pET-DEST42-ARL8B-V5-His6

    Plasmid

    #67425

    Purpose

    Expression of hArl8b with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET-DEST42-ARF1-V5-His6

    Plasmid

    #67411

    Purpose

    Expression of hArf1 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF1 (ARF1 Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. AdMKP-3 S197A

    Plasmid

    #131522

    Purpose

    Expresses MKP-3 with S197A mutation in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    changed Serine 197 to Alanine

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMC060-HisMS2_PLP_Env_pac

    Plasmid

    #155040

    Purpose

    Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Envelope Gene

    Depositor

    Paul Freemont

    Article

    Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

    Inserts

    Maturation Protein
    Coat Protein Dimer
    Envelope Gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Remove TypeIIs Restriction Sites

    Promoter

    T7

    Available since

    Sept. 9, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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