We narrowed to 77,506 results for: Mycs;
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Plasmid#188516PurposeExpresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)DepositorInsertdCas12e
UseCRISPR and LentiviralTagsNLS-FLAG-VPRExpressionMammalianMutationD659A/E756A/D922APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM4_14L
Plasmid#183063PurposePlasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locusDepositorInsertsmCherry
sfpHluorin
BM3R1-HAA1-mTurquoise2
UseSynthetic BiologyTagsmCherry and mTurquoise2ExpressionYeastMutationPromoterRET2, Synthetic promoter, and TDH3Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
attB53-Pac-tetO-mCherry-attB53
Plasmid#183612PurposeRMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).DepositorInserttetO-mCherry
UseRmceTagsExpressionMutationPromotertetOAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHis
Plasmid#175801PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH2 #7
Plasmid#166102PurposeThis plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.DepositorArticleInsertIDH2 sgRNA #7 (IDH2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROS13_IDH1 #4
Plasmid#166101PurposeThis plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.DepositorArticleInsertIDH1 sgRNA #4 (IDH1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1816
Plasmid#154320PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Phhf2-PYC-Tadh1
Plasmid#127737PurposeYeast pathway position 5. PYC transcription unit with the HHF2 promoter and ADH1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPhhf2Available sinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMuLE ENTR SV40 PuroR L5-L2
Plasmid#62165PurposeMuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloningDepositorInsertPuromycin
UseMule gateway entry vectorTagsExpressionMammalianMutationPromoterSV40Available sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppgk1-PYC-Ttpi1
Plasmid#127735PurposeYeast pathway position 5. PYC transcription unit with the PGK1 promoter and TPI1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpgk1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-de-Ppxr1-PYC-Tnat1
Plasmid#127736PurposeYeast pathway position 5. PYC transcription unit with the PXR1 promoter and NAT1 terminator.DepositorInsertpyruvate carboxylase (PYC1 Budding Yeast, Synthetic)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpxr1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pBOB-EF1-FastFUCCI-Puro
Plasmid#86849Purpose3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selectionDepositorInsertsmKO2-hCDT1(30-120)
mAG-hGEM(1-110)
pac (puromycin N-acetyl-transferase)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1 and PGKAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-oNGN2
Plasmid#198397PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.InsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationS24A, S193A, S207A, S209A, S219A, S232A, S239A, S…PromoterCAG, EF1a, and TRE3GAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO.Sox9.Puro
Plasmid#117269PurposeExpresses Sox9 and puromycin resistance gene under control of tetO sequence.DepositorInsertSox9 (Sox9 Mouse)
UseLentiviralTagsT2A and puromycin resistant cassette (C terminal …ExpressionMammalianMutationPromotertet-onAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO.Nfib.Hygro
Plasmid#117271PurposeExpresses Nfib and hygromycin resistance gene under control of tetO sequence.DepositorInsertNfib (Nfib Mouse)
UseLentiviralTagsIRES and hygromycin resistant cassette (C termina…ExpressionMammalianMutationPromotertet-onAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV_TRET_hNgn2_UBC_Puro
Plasmid#61474Purpose3rd generation lentiviral vector; TetON promoter driving human Ngn2; constitutive expression of Puromycin selection markerDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
HDAC4 Flag
Plasmid#13821PurposeMammalian expression of human histone deacetylase 4 with flag tagDepositorInsertHDAC4 (HDAC4 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-L1460P
Plasmid#69006Purposeactivating MTOR mutationDepositorInsertMTOR-L1460P (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Leucine 1460 to ProlinePromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB PUb-integrase
Plasmid#183966PurposepiggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent markerDepositorInsertPhiC31 integrase
UsePiggybac insect transgenesis destination vectorTagsExpressionMutationPromoterAedes aegypti PolyubiquitinAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Frb-v1
Plasmid#201923PurposeE. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.DepositorInsertsFrbA
FrbB
FrbC
FrbD
FrbE
UseTagsExpressionBacterialMutationPromoterT7 (modified), T7 (modified, moderate), T7 (modif…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only