We narrowed to 10,431 results for: plasmids 101

1. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

   Plasmid

   #170163

   Purpose

   Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2s-P2A-H2B-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)

   Plasmid

   #195552

   Purpose

   Expresses Thbs1 under control of short GFAP promoter

   Depositor

   Kevin Park

   Article

   Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

   Insert

   Thrombospondin-1 (Thbs1 Mouse)
   

   Use

   AAV

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   short GFAP

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CamKII-TRPV1-P2A-mCherry

   Plasmid

   #200829

   Purpose

   Transduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporter

   Depositor

   Hong Chen

   Article

   Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

   Insert

   TRPV1-P2A-mCherry (Trpv1 Rat)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CamKII

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRE-UBC-mPD-L1-T2A-GFP

   Plasmid

   #205455

   Purpose

   lentiviral plasmid for expression of mouse PD-L1

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mPD-L1 (Cd274 Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. GfaABC1D BARK D110A p2A mCherry

   Plasmid

   #117692

   Purpose

   GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   AAV

   Tags

   HA / FLAG

   Mutation

   D110A

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1a-hMLH1dn-Hygro

   Plasmid

   #195512

   Purpose

   Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn-P2A-HygroR (MLH1 Human)
   

   Use

   Lentiviral

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Human MLH1 del754-756

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNA 3C myc scfvHA

   Plasmid

   #234991

   Purpose

   Expression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins

   Depositor

   Daniel Leahy, Monica Gonzalez-Magaldi

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   NA-3C-myc-scfvHA
   

   Expression

   Mammalian

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. IR25a-T2A-QF-AD

   Plasmid

   #162526

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
   

   Expression

   Insect

   Available Since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

   Plasmid

   #170823

   Purpose

   PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d PPP2CA gRNA2
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330 PLIN2

    Plasmid

    #202196

    Purpose

    pX330 backbone expressing sgRNA to edit the C-terminus of human PLIN2

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Insert

    PLIN2 (PLIN2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_hSyn-fDIO-somQuasAr6a-EGFP-P2A-sombC1C2TG

    Plasmid

    #183537

    Purpose

    Voltage indicator

    Depositor

    Karl Deisseroth

    Article

    Fan et al Cell. 2023 Feb 2;186(3):543-559.e19. doi

    Insert

    somQuasAr6a-EGFP-P2A-sombC1C2TG
    

    Use

    AAV

    Mutation

    None

    Promoter

    hSyn

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRE-UBC-Qa1 SCT-T2A-GFP

    Plasmid

    #205453

    Purpose

    lentiviral plasmid for expression of mouse Qa1 SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    Qa1 SCT (H2-T23 Mouse)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. POLD1_pLX307

    Plasmid

    #98358

    Purpose

    Lentiviral expression of POLD1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti_PGK-Cas9-P2A-HygR

    Plasmid

    #164135

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-P3-IgKL-mNG

    Plasmid

    #183465

    Purpose

    To produce AAV with the P3 promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    IgKL-mNeonGreen
    

    Use

    AAV

    Tags

    IgKL secretory signal

    Available Since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-MPRA-MluI-SpeI-EcoRI

    Plasmid

    #190196

    Purpose

    Empty vector for inserting MPRA libraries into AAV

    Depositor

    Hyejung Won

    Article

    McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

    Type

    Empty backbone

    Use

    AAV; Massively parallel reporter assay (mpra)

    Expression

    Mammalian

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-H1-shScramble-hsynapsin-EGFP

    Plasmid

    #220795

    Purpose

    control shRNA plasmid with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CBh-IgKL-mNG

    Plasmid

    #183467

    Purpose

    To produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

    Depositor

    Hajime Hirase

    Article

    Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

    Insert

    IgKL-mNeonGreen
    

    Use

    AAV

    Tags

    IgKL secretory signal

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

    Plasmid

    #171150

    Purpose

    Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2f-P2A-H2B-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

    Plasmid

    #214732

    Purpose

    Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

    Depositor

    Félix Leroy

    Article

    de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

    Insert

    anti-Crh shRNA / mCherry
    

    Use

    AAV

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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