We narrowed to 4,572 results for: TIL
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Plasmid#103090PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertC4ZA16(Cas9 coding gene from Eubacterium rectale (strain ATCC 33656 / VPI 0990))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUPI hT5Cyp-H126Q
Plasmid#80281PurposeExpresses human TRIM5 CypA fusion with H126Q mutation in CypADepositorUseLentiviralExpressionMammalianMutationH126Q mutation in CypAAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSC-mTDG-K341R-IntE2
Plasmid#52269PurposeBacterial expression of mouse TDG-K341R C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInsertTDG (Tdg Mouse)
TagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK341R, SUMO acceptor site mutationPromoterT7Available SinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKCsub(T/A)-RLuc8N-NES
Plasmid#138299PurposeEncodes negative-control substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKCsub(T/A)-RLuc8N-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pFSW Doc2beta 6X mutant
Plasmid#128816PurposeTo make virus expressing Doc2beta 6X mutantDepositorInsertDoc2beta (Doc2b Rat)
UseLentiviralTagsIRES-EGFPMutationD163A, D218A, D220N, D303A, D357A, D359APromoterhSyn (human synapsin I promoter)AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STING
Plasmid#102586PurposeLentivector to express STING and mTagBFPDepositorAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP
Plasmid#118594PurposeParental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoterDepositorInsertsgRNA/Puro-T2A-BFP
UseLentiviralExpressionMammalianPromoterEF1AlphaAvailable SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-hCD8
Plasmid#153417PurposeNFAT-driven ZsGreen-1 reporter gene with human CD8ab geneDepositorInsert8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
UseRetroviralExpressionMammalianAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDG459
Plasmid#100901PurposeSpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-PuroRExpressionMammalianPromoterCBh and U6Available SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-STING_gRNA3
Plasmid#127640PurposeKnock-out of human STINGDepositorInsertSTING (STING1 Human)
UseLentiviralAvailable SinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only