We narrowed to 18,937 results for: Tec

1. psiCHECK-2-TGFBR2-wt_3'UTR

   Plasmid

   #31882

   Depositor

   Robert Blelloch

   Article

   Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

   Insert

   TGFBR2 wild-type 3'UTR (TGFBR2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pAM4951 (pCV0063)

   Plasmid

   #120084

   Purpose

   Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

   Depositor

   Susan Golden

   Article

   Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

   Insert

   Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
   

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pREMI-ZA

   Plasmid

   #183736

   Purpose

   Plasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffii

   Depositor

   Jun Ishii

   Article

   Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

   Insert

   Sh ble
   

   Use

   Random insertional gene deletion in komagataella …

   Promoter

   TEF1 promoter

   Available Since

   March 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST- IP2.0(codon for C.elegans)

   Plasmid

   #109216

   Purpose

   IP2.0 for C. elegans (Codon optimization and introns).

   Depositor

   Takeshi Ishihara

   Article

   Hara-Kuge et al PLoS One. 2018 Apr 25;13(4):e0194707. do

   Insert

   IP2.0 (Inverse Pericam 2.0)
   

   Tags

   His-tag

   Expression

   Worm

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. sfGFP-SasG(8G)-mCherry

   Plasmid

   #206950

   Purpose

   Mammalian expression of heterobifunctional SasG linkers (8G)

   Depositor

   Gil Westmeyer

   Article

   Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

   Insert

   sfGFP-SaSasG (8G)-mCherry
   

   Tags

   mCherry and sfGFP

   Expression

   Mammalian

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCosSAD1AprR

   Plasmid

   #190786

   Purpose

   An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination

   Depositor

   Jan Kormanec

   Article

   Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(

   Inserts

   upstream region of the aur1 cluster for auricin
   ampramycin resistance cassette
   downstream region of the aur1 cluster for auricin
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ZIKV_Trigger_27B

   Plasmid

   #75008

   Purpose

   Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

   Depositor

   James Collins, Alexander Green

   Article

   Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

   Insert

   ZIKV_Trigger_27B
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196079

   Purpose

   Constitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

   Plasmid

   #196085

   Purpose

   Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting Adam10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO.1P shFXN_1

    Plasmid

    #102970

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_1 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GEV 1

    Plasmid

    #12615

    Depositor

    John Louis

    Article

    Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

    Insert

    GB1 domain of protein G as N terminal tag, with Bcl2 as insert (Bcl2 Mouse)
    

    Tags

    G protein and His

    Expression

    Bacterial

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLINE1-scFvFc

    Plasmid

    #206020

    Purpose

    An intact type LINE-1 vector plasmid possessing the scFv-Fc expression unit

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit
    

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-RapR-FAK-YM-KD

    Plasmid

    #25929

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse, Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This pEGFP-RapR-FAK-YM-KD plasmid contains the FA…

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIMVAd39T

    Plasmid

    #98303

    Purpose

    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

    Expression

    Yeast

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

    Plasmid

    #191397

    Purpose

    sgRNA targeting alpha-satellites on human chromosome 9

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    sgRNA targeting alpha satellites on human chromosome 9
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV_APOBEC1-UdgX-nCas9

    Plasmid

    #163531

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    APOBEC1-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-RIPK3[S227D]-FLAG

    Plasmid

    #199320

    Purpose

    mutated phosphorylation site (MLKL interaction permanent active)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 227 to Aspartic Acid

    Available Since

    May 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-RIPK3[S227A]-FLAG

    Plasmid

    #199318

    Purpose

    mutated phosphorylation site (MLKL interaction inhibited)

    Depositor

    Adrian Press

    Article

    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

    Insert

    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 227 to Alanine

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB3051(gRNA X-3 XI-2 XII-2)

    Plasmid

    #73293

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1P shFXN_2

    Plasmid

    #102971

    Purpose

    Suppression of FXN

    Depositor

    Richard Possemato

    Article

    Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

    Insert

    shFXN_2 (FXN Human)
    

    Use

    Lentiviral and RNAi

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge