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pLEX_970_puro_DEST_V5_LUC

Plasmid

#45290

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab1 DN

Plasmid

#37682

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab1 (Rab1 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

S25N (dominant negative)

Promoter

GAL4

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-HA-SH3BP4 W92A

Plasmid

#46893

Purpose

expresses HA tagged SH3BP4 W92A mutant in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 W92A (SH3BP4 Human)

Tags

HA

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab7 DN

Plasmid

#37703

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T22N (dominant negative)

Promoter

GAL4

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pTJK6 (miR-513a WT OE in pWCC52)

Plasmid

#254385

Purpose

Express human miR-513a

Depositor

Curt Civin

Article

Kim et al FEBS Open Bio. 2026 Mar 8. doi: 10.1002/

Insert

miR-513a-1 (MIR513A1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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Modified pYTK048

Plasmid

#254021

Purpose

It was constructed by reversing the orientation of the BsaI sites in the original pYTK048 and is used for the construction of connectors.

Depositor

Dae-Hee Lee

Article

Seong et al Trends Biotechnol. 2026 Jan 14:S0167-779

Insert

Spacer

Use

Synthetic Biology

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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[#GS31-LV] CMV-MitoTRACER-Donor

Plasmid

#253759

Purpose

Lentiviral MitoTRACER donor construct under CMV Promoter

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER Donor

Tags

GFP11 and HA Tag

Promoter

CMV

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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[#GS32-LV] EF1a-MitoTRACER-Donor

Plasmid

#253760

Purpose

Lentiviral MitoTRACER donor construct under EF1a Promoter

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Tags

GFP11 and HA Tag

Promoter

EF1a

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)-FLEx(loxP)-ARch3.0:EYFP

Plasmid

#248667

Purpose

Expresses Archaerhodopsin3 tagged to eYFP in Cre-expressing cells under the CAG promoter for neuronal inhibition.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

Arch3.0-EYFP

Use

AAV

Tags

eYFP

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG(del)--FLEx(loxP)-splitTVA:HA

Plasmid

#248665

Purpose

Helper virus for monosynaptic tracing; Expresses TVA and an HA tag in Cre-expressing cells under the CAG promoter. To be co-injected with Rabies Glycoprotein (G) viruses.

Depositor

Gordon Fishell

Article

Mazo et al Res Sq [Preprint]. 2025 Jun 12:rs.3.rs-6

Insert

TVA

Use

AAV

Tags

HA

Expression

Mammalian

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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TEVESNp_pRK793

Plasmid

#247042

Purpose

pRK793 (from David Waugh, Addgene plasmid #8827) backbone with ENLYFES cut site and TEVESNp insert.

Depositor

Carl Denard

Article

Nelson et al ACS Synth Biol. 2025 Jun 20;14(6):2241-2

Insert

MBP-ENLYFES-7xHIS-TEVESNp

Tags

His and MBP

Expression

Bacterial

Mutation

S120C, D148R, Tl73A, N174R, N176 V, Nl77K, G213A,…

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8-MET15

Plasmid

#207032

Purpose

Expression of 3HA-Atg8.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Promoter

pATG8

Available Since

Jan. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-miRFP703-dSal-p53NT(1-97)-S15D-hCRY2-NLS

Plasmid

#241842

Purpose

Expresses an actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

N-terminus (1-97 aa) containing S15D

Promoter

CAGGS promoter

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-d7F

Plasmid

#245784

Purpose

Expression of mutated bovine arrestin-3 (deleted residues 175, 177, 178, 182-185) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

deleted residues 175, 177, 178, 182-185

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆11cag

Plasmid

#245370

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆11cag (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

11-bp deletion, H96Wfs*4, plus 3 synonymous mutat…

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pZNpF-∆7cg

Plasmid

#245371

Purpose

Dual luciferase (nano, firefly) with Zfp423 5' end fused to NanoLuciferase

Depositor

Bruce Hamilton

Article

Concepcion et al bioRxiv [Preprint]. 2025 Jun 2:2025.05.3

Insert

Zfp423-∆7cg (Zfp423 Mouse)

Use

Luciferase and Synthetic Biology

Tags

NanoLuciferase

Expression

Mammalian

Mutation

7-bp deletion, H96Vfs*29, plus 2 synonymous mutat…

Promoter

EF1a

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EBFP2-K2K1

Plasmid

#242820

Purpose

Encodes human kindlin2 containing kindlin1 F3 domain and N-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Kindlin2 (FERMT2 Human)

Expression

Mammalian

Mutation

Kindlin2 chimera containing the F3 domain of kind…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mE-GFP2A

Plasmid

#236247

Purpose

Middle entry fluorescent GFP reporter with a viral 2A peptide for use with the Gateway cloning system; allows 3' transgene to be transcribed on the same mRNA but translated separately

Depositor

James Amatruda, Genevieve Kendall

Article

Kendall et al eLife. 2018 Jun 5;7. pii: 33800. doi: 10

Insert

eGFP

Expression

Mammalian

Mutation

Added a viral2A sequence with no stop codon

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β6β1-EGFP

Plasmid

#242829

Purpose

Encodes human integrin β6 chimera containing integrin β1 cytoplasmic domain and C-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 6, transcript variant 1 (ITGB6 Human)

Expression

Mammalian

Mutation

Integrin β6 chimera containing the cytoplasmic ta…

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(WT)-EGFP

Plasmid

#196233

Purpose

EGFP fused to the C-terminus of Af1521 (WT) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(WT)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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