We narrowed to 1,251 results for: Lyn

1. pEM-Cas9HF1

   Plasmid

   #89961

   Purpose

   Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   Cas9HF1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMART-ala10

   Plasmid

   #87135

   Purpose

   NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Inserts

   NADPH Alanine Dehydrogenase (ald Synthetic)
   alanine exporter (alaE Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D196A, L197R

   Promoter

   waaHp from E. coli as part of operon with ald* an…

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRII-Topo Gp88 in situ probe

   Plasmid

   #45628

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   Gpr88 in situ probe (Gpr88 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains bp# 2742-3255 of AB042408.1 or …

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCRII-Topo Epha3 in situ probe

   Plasmid

   #45627

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   Epha3 in situ probe (Epha3 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains bp# 3179-3773 of NM_010140

   Promoter

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCRII-Topo Hspb3 in situ probe

   Plasmid

   #45629

   Depositor

   Jeffrey Macklis

   Article

   Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

   Insert

   Hspb3 in situ probe (Hspb3 Mouse)
   

   Use

   In situ

   Tags

   Expression

   Mutation

   fragment contains nt 30-661 of Hspb3 mRNA sequenc…

   Promoter

   Available since

   June 4, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pHAND1-AM-Tag-PGK-Neo

   Plasmid

   #223216

   Purpose

   Targeting plasmid for inserting a C-terminal AM-Tag in the human HAND1 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

   Insert

   HAND1 (HAND1 Human)
   

   Use

   Cre/Lox; Human targeting

   Tags

   AM-Tag

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAND1-T2A-dTom-PGK-Neo

   Plasmid

   #209838

   Purpose

   Tomato reporter plasmid targeting the human HAND1 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al Stem Cell Res. 2024 Mar 4;77:103342. doi

   Insert

   HAND1-T2A-dTomato-PGK-Neo-HAND1 (HAND1 Human)
   

   Use

   Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSOX17-T2A-dTom-PGK-Neo

   Plasmid

   #223190

   Purpose

   Tomato reporter plasmid targeting the human SOX17 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

   Insert

   SOX17-T2A-dTomato-PGK-Neo-SOX17 (SOX17 Human)
   

   Use

   Cre/Lox; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFOXA2-T2A-mTagBFP2-PGK-Neo

   Plasmid

   #223191

   Purpose

   BFP reporter plasmid targeting the human FOXA2 gene

   Depositor

   Matthew Birket

   Article

   Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

   Insert

   FOXA2-T2A-mTagBFP2-PGK-Neo-FOXA2 (FOXA2 Human)
   

   Use

   Cre/Lox; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-FLAG-HAND1-T2A-mTagBFP2

    Plasmid

    #223192

    Purpose

    Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

    Depositor

    Matthew Birket

    Article

    Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

    Insert

    HAND1 (HAND1 Human)
    

    Use

    Promoterless entry vector for gateway cloning

    Tags

    FLAG and T2A-mTagBFP2

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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