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pUASP YFP Rab32 WT

Plasmid

#37738

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
pAc5.1B-EGFP-DmHPat

Plasmid

#25029

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

HPat (Patr-1 Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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Rae1-pDONR221

Plasmid

#124240

Purpose

Gateway entry clone of Drosophila Rae1 cDNA.

Depositor

Peter Vilmos

Article

Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

Insert

Drosophila Rae1 (Rae1 Fly)

Expression

Insect

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAP2

Plasmid

#41755

Depositor

Jeff Gelles

Article

Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

kinesin heavy chain (Khc E. coli, Fly)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; C45S C338S K349C C451S

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00334

Plasmid

#34457

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG11491 (br Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pUASP dsRed Rab10 WT

Plasmid

#44828

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
Dscam1_7.27.25 EC10-Fc-His

Plasmid

#72188

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.25 EC10-AP-His

Plasmid

#72062

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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KHBD00143

Plasmid

#34297

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1759 (cad Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
CJ29 (let-7 perfect site)

Plasmid

#17766

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

let-7 perfect site (let-7 Fly)

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pUASt-SIK3 S563A

Plasmid

#52976

Purpose

insect expression of SIK3 S563A PKA phosphorylation defective mutant

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

S563A PKA phosphorylation defective

Promoter

Hsp70

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-attB-yki:V5-S111A

Plasmid

#85619

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S111A

Tags

V5

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S250A

Plasmid

#85618

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S250A

Tags

V5

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00133

Plasmid

#34287

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2047 (ftz Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac His HA GNU WT

Plasmid

#112994

Purpose

Baculovirus expression of His/HA-tagged Drosophila gnu

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

gnu (gnu Fly)

Tags

His/HA

Expression

Insect

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-56

Plasmid

#92260

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

HA

Expression

Insect

Promoter

Ac5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-Patronin CC 638–1457

Plasmid

#59051

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 776–1457

Plasmid

#59052

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
gRNA[bTub].1026C

Plasmid

#112691

Purpose

express gRNA targeting bTub under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub]

Use

CRISPR

Promoter

dU6-3

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTGC-U6.2

Plasmid

#112809

Purpose

PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

U6:2-gRNAcore

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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