3,646 results

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Dscam1_7.27.25 EC10-Fc-His

Plasmid

#72188

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.25 EC10-AP-His

Plasmid

#72062

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.25 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.25

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pUASt-SIK3 S563A

Plasmid

#52976

Purpose

insect expression of SIK3 S563A PKA phosphorylation defective mutant

Depositor

Marc Montminy, Biao Wang

Article

Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

Insert

SIK3 (Sik3 Fly)

Tags

HA

Expression

Insect

Mutation

S563A PKA phosphorylation defective

Promoter

Hsp70

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-attB-yki:V5-S111A

Plasmid

#85619

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S111A

Tags

V5

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S250A

Plasmid

#85618

Purpose

Expresses V5 tagged mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S250A

Tags

V5

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00133

Plasmid

#34287

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2047 (ftz Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac His HA GNU WT

Plasmid

#112994

Purpose

Baculovirus expression of His/HA-tagged Drosophila gnu

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

gnu (gnu Fly)

Tags

His/HA

Expression

Insect

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-56

Plasmid

#92260

Purpose

Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

Depositor

Marina Chekulaeva, Roy Parker

Article

Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

Insert

dGW182 (gw Fly)

Tags

HA

Expression

Insect

Promoter

Ac5

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-GFP-Patronin CC 638–1457

Plasmid

#59051

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-GFP-Patronin CC 776–1457

Plasmid

#59052

Purpose

for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Tags

eGFP

Expression

Bacterial and Insect

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
gRNA[bTub].1026C

Plasmid

#112691

Purpose

express gRNA targeting bTub under dU6-3 promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

U6.3-gRNA[bTub]

Use

CRISPR

Promoter

dU6-3

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTGC-U6.2

Plasmid

#112809

Purpose

PCR template vector for amplifying gRNAcore-pU6.3 promoter fragment

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Insert

U6:2-gRNAcore

Expression

Bacterial

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pUASP YFP Rab18 WT

Plasmid

#37717

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab18 (Rab18 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab7 CA

Plasmid

#37704

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab7 (Rab7 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q67L (constitutive active)

Promoter

GAL4

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
KHBD00664

Plasmid

#39719

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG18023 (Eip78C Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00250

Plasmid

#34383

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7199 (Hr78 Fly)

Use

Gateway Cloning

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pGEM-T Dmel Or56a

Plasmid

#59663

Purpose

Probe for Drosophila melanogaster Or56a expression

Depositor

Richard Axel, Leslie Vosshall

Article

Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

Insert

Or56a (Or56a Fly)

Expression

Bacterial

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac Dual PNG-FLAG PLU-HIS

Plasmid

#113173

Purpose

Baculovirus expression of PNG and PLU

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

PNG, PLU

Use

Baculovirus

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 dSesn (1187)

Plasmid

#66919

Purpose

bacterial expression of dSesn

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

Insert

Sesn (Sesn Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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PGRP-LCa

Plasmid

#21059

Depositor

Johann Deisenhofer

Article

Chang et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

PGRP-LCa (PGRP-LF Fly)

Tags

His

Expression

Insect

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only