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Plasmid#120715PurposeExpresses early endosome-targeted recruiter protein in mammalian cells & for virus productionDepositorInsertRab5a
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationAmino acids 2-215PromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
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JDW 743 (pDONR221-EYFP-hKRAS4B-WT)
Plasmid#156402PurposeGateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codonDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseGateway cloningTagsEYFPExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)
Plasmid#156411PurposepCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4ADepositorInserthuman wild type KRAS4A-WT (KRAS Human)
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 763 (pCS2-EYFP-hsKRAS4B-WT)
Plasmid#156408PurposepCS2 CMV expression vector driving EYFP fused human KRAS4BDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)
Plasmid#156405PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codonDepositorInserthuman wild type KRAS4A-WT (KRAS Human)
UseGateway cloningTagsmScarlet-IExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 742 (pDONR221-EGFP-KRAS4B-G12V)
Plasmid#156403PurposeGateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codonDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseGateway cloningTagsEGFPExpressionMutationmutant KRAS4B-G12VPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)
Plasmid#156410PurposepCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4A-G12VDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseTagsmScarlet-IExpressionMammalianMutationmutant KRAS4A-G12VPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)
Plasmid#156409PurposepCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12DDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseTagsmTagBFP2ExpressionMammalianMutationmutant KRAS4A-G12DPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 762 (pCS2-EGFP-hsKRAS4B-G12V)
Plasmid#156407PurposepCS2 CMV expression vector driving EGFP fused human KRAS4B-G12VDepositorInserthuman mutant KRAS4B-G12V (KRAS Human)
UseTagsEGFPExpressionMammalianMutationmutant KRAS4B-G12VPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-EGFP (LTH1384)
Plasmid#185488PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(BCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-EGFP (LTH1430)
Plasmid#185485PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(PDCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)
Plasmid#185490PurposeCMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorInserthuman codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationH840APromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)
Plasmid#156406PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12V (KRAS Human)
UseGateway cloningTagsmScarlet-IExpressionMutationmutant KRAS4A-G12VPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(SCA)-BPNLS(SV40)-P2A-EGFP (LTH1378)
Plasmid#185484PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(SCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-EGFP (LTH1441)
Plasmid#185486PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(PCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-EGFP (LTH1380)
Plasmid#185487PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and P2A-eGFPDepositorInserthuman codon usage NLS-anCas(FCA)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-P2A-eGFP and NLSExpressionMammalianMutationPromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)
Plasmid#185492PurposeCMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorInserthuman codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationD10APromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)
Plasmid#156413Purposekdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sitesDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseZebrafish transgenesisTagsEYFPExpressionMutationPromoterkdrlAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)
Plasmid#156404PurposeGateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codonDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseGateway cloningTagsmTagBFP2ExpressionMutationmutant KRAS4A-G12DPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Eef25UTR-TOPmut-renilla
Plasmid#38236DepositorInsertEef2_TOPm_5UTR/Renilla (Eef2 Mouse)
UseLuciferaseTagsExpressionMammalianMutationThe first nucleotides of the 5' UTR were cha…PromoterEndogenous Eef2Available sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pACYC-TtCas6-4xcrRNA4.5
Plasmid#127764PurposeFor expression of T. thermophilus Csm complex in E. coli (crRNA)DepositorInsertsSynthetic CRISPR array containing four identical T. thermophilus spacers
Cas6A
UseTagsExpressionBacterialMutationCodon optimized for expression in E. coliPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR 19b-3p
Plasmid#82442PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-19b-3pDepositorInserthsa-miR-19-3p
UseTagsExpressionMutationComplementary region for hsa-miR-19-3p as sensorPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTJK511
Plasmid#121468Purpose2µm HIS3 CNB1mutEF4DepositorInsertCNB1mutEF4 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 144 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK510
Plasmid#121465Purpose2µm HIS3 CNB1mutEF3DepositorInsertCNB1mutEF3 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 103 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK509
Plasmid#121464Purpose2µm HIS3 CNB1mutEF2DepositorInsertCNB1mutEF2 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 66 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK508
Plasmid#121463Purpose2µm HIS3 CNB1mutEF1DepositorInsertCNB1mutEF1 (CNB1 Budding Yeast)
UseYeast high copy 2 micron vectorTagsExpressionMutationChanged aspartic acid 34 to alaninePromoterEndogenous genomic promoter regionAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5-FLAG-p14
Plasmid#42330DepositorInsertp14 (LAMTOR2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)
Plasmid#156415Purposekdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sitesDepositorInserthuman mutant KRAS4A-G12D (KRAS Human)
UseZebrafish transgenesisTagsmTagBFP2ExpressionMutationmutant KRAS4A-G12DPromoterkdrlAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)
Plasmid#185491PurposeCMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorInserthuman codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationD10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only