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Plasmid#120048PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE13
Plasmid#90348PurposeLXR - Liver X receptor gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterLXRAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE15
Plasmid#90354PurposeRARA - Retinoic acid receptor gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterRAREAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-05
Plasmid#119984PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIC-SM-Myc
Plasmid#111189PurposeHigh affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)DepositorTypeEmpty backboneUseToxoplasma gondii expressionTagsSpaghetti Monster Myc TagExpressionMutationPromoterEndogenousAvailable sinceAug. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMaCTag-Z02
Plasmid#120045PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with TagBFPDepositorInsertTagBFP
UsePcr templateTagsTagBFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE14
Plasmid#90349PurposeVDR - Vitamin D receptor gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterVDRAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFPI7
Plasmid#83555PurposeUsed to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.DepositorInsertCUP1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterCUP1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4-3xP3-RFP
Plasmid#127556PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIC-SM-HA
Plasmid#111188PurposeHigh affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)DepositorTypeEmpty backboneUseToxoplasma gondii expressionTagsSpaghetti Monster HA TagExpressionMutationPromoterEndogenousAvailable sinceAug. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAf-CRISPR-yA
Plasmid#191015PurposeCRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.DepositorInsertyA
UseCRISPRTagsExpressionMutationPromoterAspergillus flavus U6Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
hum sgk promoter F4R4
Plasmid#83426Purposepromoter reporter construct to test 5' flanking sequencesDepositorInserthuman Sgk1 gene 5' flanking seq + 117 nt of the 5' UTR (SGK1 Human)
UseLuciferaseTagsLuciferaseExpressionMutationPromotersgkAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
PL552
Plasmid#68407PurposeDonor empty vector with floxed puromycin resistance gene expression cassetteDepositorTypeEmpty backboneUseCre/LoxTagsExpressionMammalianMutationPromotermouse PGK promoterAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPGK1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P34
Plasmid#120030PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xFLAGDepositorInsert3xFLAG
UsePcr templateTags3xFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P05
Plasmid#120016PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with eGFPDepositorInserteGFP
UsePcr templateTagseGFPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P28
Plasmid#120039PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 3xHADepositorInsert3xHA
UsePcr templateTags3xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMBA328
Plasmid#214748PurposeMedium copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA333
Plasmid#214745PurposeMedium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
TrpLE-M-nMSP1E3D1
Plasmid#179949PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.DepositorInsertTrpLE-M-nMSP1E3D1
UseTagsTrpLEExpressionBacterialMutationNative Trp residues have been mutated to Phe resi…PromoterT7Available sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
L0-I35 p35S-XVE-pEstradiol GG0-GG1
Plasmid#195904PurposeLevel 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)DepositorInsertp35S-XVE-LexA-p35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKAd5f11pES-PmeI
Plasmid#122698PurposeTo generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.DepositorTypeEmpty backboneUseAdenoviralTagsExpressionMutationPromoterAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGM1202
Plasmid#69615Purposeinducible gene expression in Streptomyces, C-terminal His-tag encoding sequenceDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
UseTagsExpressionMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_D-CmYLCV-NbGP41P-sfGFP-VP64-GB1
Plasmid#202010PurposeMoclo level 1 - Module D, Promoter: CmYLCV, Gene: NbGP41P-sfGFP-VP64-GB1, Terminator: ZmUBIDepositorInsertNbGP41P-sfGFP-VP64-GB1
UseSynthetic Biology; Moclo level 1 vectorTagsGB1 and sfGFPExpressionMutationPromoterCmYLCVAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
gRNAy
Plasmid#168294PurposeExpresses gRNA targeting the yellow gene in DrosophilaDepositorInsertU6.3-gRNA[y]-scaffold
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-13
Plasmid#119992PurposeTemplate for C-terminal PCR tagging of mammalian genes (without selection) with mCherryDepositorInsertmCherry
UsePcr templateTagsmCherryExpressionMutationPromoterNoneAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P22
Plasmid#120033PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAPDepositorInsertSNAP
UsePcr templateTagsSNAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTDH3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P07
Plasmid#124788PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB5A
Plasmid#83556PurposeUsed to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.DepositorInsertGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P33
Plasmid#120018PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAGDepositorInsertFLAG
UsePcr templateTagsFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
P1-EYFP-pA (Construct 5)
Plasmid#55197PurposeSynthetic promoter responsive to gRNA1DepositorInsertEYFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNSA-ARS-CRISPR-BlastR
Plasmid#101009PurposeExpresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.DepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P27
Plasmid#120038PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 1xHADepositorInsert1xHA
UsePcr templateTags1xHAExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRvCAHS3-CAHS3-mEGFP
Plasmid#205006PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.DepositorInsertRvCAHS3-mEGFP with 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-FLPo-3xP3-RFP
Plasmid#127561PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.DepositorInsertFLPo
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRvSAHS1-SAHS1-mEGFP
Plasmid#205007PurposeThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.DepositorInsertRvSAHS1-mEGFP with 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC_mClover3_BSDminus_F0
Plasmid#184165PurposeDonor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.DepositorInsertsmClover3
bsd
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP95
Plasmid#83550PurposeUsed to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.DepositorInsertPDA1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPDA1Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
JD141
Plasmid#229487PurposeEf1a promoter driving expression of adenoviral L4-22k/33k genesDepositorInsertL4-22k/33k
UseAAV and AdenoviralTagsExpressionMammalianMutationPromoterEf1aAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDS24
Plasmid#58952PurposeEncodes synthetic mitochondrial gene ARG8m in place of COX3 coding sequence. Flanked by mtDNA sequences allowing integration into rho+ mtDNA after transformation.DepositorInsertMitochondrially coded Arg8 protein
UseTagsExpressionMutationCoding sequence synthetic in S. cerevisiae mitoch…Promotern/aAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKL2013
Plasmid#172182PurposeA CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.DepositorInsertmCherry
UseCRISPRTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z22
Plasmid#120065PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAPDepositorInsertSNAP
UsePcr templateTagsSNAPExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGM51559
Plasmid#153226PurposeControl plasmid for knockout of TRY and CPC genesDepositorInsertCas9, guide RNA for AP3, FAST marker
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-intron
Plasmid#177804PurposeAn intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.DepositorInsertintron
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-sfGFP-3xP3-RFP
Plasmid#127557PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertsfGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV+ plasmid
Plasmid#169740PurposeUnregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.DepositorInserteGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpCMV-tetO2Available sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal80-3xP3-RFP
Plasmid#127562PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal80
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only