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Showing: 701 - 720 of 3893 results

  6. p1D4-hrGFP II

    Plasmid
    #71317
    Purpose
    expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.
    Depositor
    Belinda Chang
    Article
    Morrow et al Plasmid. 2010 Nov;64(3):162-9. doi: 10.1
    Type
    Empty backbone
    Use
    Tags
    1D4 epitope (TETSQVAPA) and IRES-hrGFPII
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  7. pMV_AA091

    Plasmid
    #216106
    Purpose
    CRISPRa, gRNA expression with modified trRNA to recruit activators via PP7 tags (guide only)
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    gRNA expression with modified trRNA to recruit activators via PP7 tags
    Use
    CRISPR and Lentiviral; Assembled vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216106-plasmid-map-sequence-id-431410

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  8. pcDNA5-FRT-TO-Nsp13-HF_GC3opt

    Plasmid
    #157716
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157716-plasmid-map-sequence-id-307912

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  9. pcDNA5-FRT-TO-Nsp8-HF_GC3opt

    Plasmid
    #157699
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157699-plasmid-map-sequence-id-307863

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  10. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

    Plasmid
    #157693
    Purpose
    mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Article
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Insert
    Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
    Use
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157693-plasmid-map-sequence-id-307835

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  14. pIA030

    Plasmid
    #200424
    Purpose
    thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    PAmCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Available since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    200424-plasmid-map-sequence-id-405349

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  15. pelBHisTEV

    Plasmid
    #157741
    Purpose
    empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    PelB signal sequence + His10-tag + TEV protease c…
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157741-plasmid-map-sequence-id-334005

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Showing: 701 - 720 of 3893 results