Skip to main content
Addgene
Showing: 701 - 720 of 65319 results

  1. pAC-ZETAipi

    Plasmid
    #53284
    Purpose
    Contains crtE, crtB, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and pds (crtP) gene of Synechococcus PCC7942, and thereby produces zeta-carotene in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    pds
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    lac
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53284-plasmid-map-sequence-id-83691

    Enlarge

  2. pAC-ZETA

    Plasmid
    #53316
    Purpose
    Contains Erwinia herbicola Eho10 genes crtE and crtB, together with the pds (crtP) gene of Synechococcus PCC7942 fused to the lacZ gene at the N terminus. Produces zeta-carotene in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    pds
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    lazZ
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53316-plasmid-map-sequence-id-83035

    Enlarge

  3. pAC-NEUR

    Plasmid
    #53271
    Purpose
    Contains the Erwinia herbicola (Pantoea agglomerans) Eho10 genes crtE and crtB, and the Rhodobacter capsulatus crtI gene and thereby produces neurosporene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtI
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53271-plasmid-map-sequence-id-82987

    Enlarge

  6. pAL942

    Plasmid
    #119721
    Purpose
    precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Alanine 248 to Threonine, Alanine 188 to Cysteine…
    Promoter
    T7
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119721-plasmid-map-sequence-id-232465

    Enlarge

  9. pAC-LYC

    Plasmid
    #53270
    Purpose
    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53270-plasmid-map-sequence-id-82990

    Enlarge

  10. pAC-CANTHipi

    Plasmid
    #53301
    Purpose
    Contains crtE, idi, crtI, crtY, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and a crtO cDNA of Haematococcus pluvialis fused to a Trc promoter. Produces canthaxanthin in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtO
    Use
    Low copy number bacterial cloning vector
    Tags
    6His
    Expression
    Mutation
    Promoter
    Trc
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53301-plasmid-map-sequence-id-99820

    Enlarge

  11. pAC-ZEAXipi

    Plasmid
    #53287
    Purpose
    Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtE, idi, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53287-plasmid-map-sequence-id-82991

    Enlarge

  14. pAC-BETA-At

    Plasmid
    #53288
    Purpose
    Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and an lcyB cDNA of Arabidopsis thaliana. Use with pY2F to produce alpha-carotene in E. coli.
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu
    Insert
    lcyB (LYC Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53288-plasmid-map-sequence-id-83282

    Enlarge

  15. pAC-aZEA

    Plasmid
    #53286
    Purpose
    Contains crtE, and crtB genes of Erwinia herbicola (Pantoea agglomerans) Eho10, crtI gene of Rhodobacter capsulatus, and lcyE cDNA of Arabidopsis thaliana and produces alpha-zeacarotene in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    lcyE (LUT2 Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    lac
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53286-plasmid-map-sequence-id-83099

    Enlarge

  16. pAPi

    Plasmid
    #127548
    Purpose
    Empty control for pGAPi-based RNAi silencing in tandem with APT transcript segment
    Depositor
    Luis Vidali
    Article
    Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00
    Insert
    Adenine Phosphorybosyltransferase transcript segment
    Use
    RNAi; Empty control
    Tags
    Expression
    Mutation
    Promoter
    Ubiquitin
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    127548-plasmid-map-sequence-id-247702

    Enlarge

  17. pAC-BETA

    Plasmid
    #53272
    Purpose
    Contains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1996 Sep;8(9):1613-26.
    Insert
    crtE, crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53272-plasmid-map-sequence-id-83327

    Enlarge

  18. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

    Enlarge

  19. HF-PX459 (V2)

    Plasmid
    #118632
    Purpose
    Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
    Depositor
    Mike McGrew
    Article
    Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10
    Insert
    hSpCas9-HF1-2A-Puro V2.0
    Use
    CRISPR
    Tags
    2A-Puro and 3XFLAG
    Expression
    Mammalian
    Mutation
    Asparagine 497 to Alanine, Arginine 661 to Alanin…
    Promoter
    Cbh
    Available since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    118632-plasmid-map-sequence-id-230217

    Enlarge

  20. pRN3P_T3_ABE8e_IVT

    Plasmid
    #201676
    Purpose
    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.
    Depositor
    Timo Otonkoski
    Article
    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72
    Insert
    ecTadA(8e)-nSpCas9
    Use
    CRISPR; Vector for in-vitro transcription
    Tags
    Expression
    Mutation
    Promoter
    T3 promoter
    Available since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    201676-plasmid-map-sequence-id-406944

    Enlarge

Showing: 701 - 720 of 65319 results