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Cbh_v5 AAV-ABE N-terminal

Plasmid

#137177

Purpose

AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-ABE N-terminal

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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lenti U6-sgRNA/EF1a-mCherry

Plasmid

#114199

Purpose

Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

sgRNA scaffold

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

U6

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pVITRO1-dV-IgG1/κ

Plasmid

#52213

Purpose

Human immunoglobulin gamma 1 antibody expression vector (kappa light chain) without variable regions. Enables Colony-PCR screening of false-positive (PCR vector template) colonies

Depositor

Andrew Beavil

Article

Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

Inserts

Human immunoglobulin gamma 1 constant region
Human immunoglobulin kappa constant region

Expression

Mammalian

Mutation

Deleted Variable region

Promoter

Mouse Elongation Factor 1 Alpha and Rat Elongatio…

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP36

Plasmid

#22579

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP36 (USP36 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-GFP-αTAT1

Plasmid

#27099

Depositor

Maxence Nachury

Article

Shida et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

α-Tubulin K40 acetyltransferase1 (ATAT1 Human)

Tags

GFP, S-tag, and TEV site

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Article

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Mutation

*see comment below

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut1

Plasmid

#18085

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut1 (SLC2A1 Human)

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-ABE C-terminal

Plasmid

#137178

Purpose

AAV genome: expresses the C-terminal of v5 AAV-ABE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

CMV

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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FU-tetO-Gateway

Plasmid

#43914

Depositor

John Gearhart

Article

Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

Type

Empty backbone

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

6xHis and V5

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV PRDM16

Plasmid

#15504

Depositor

Bruce Spiegelman

Article

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (isoform 4) (Prdm16 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pET His6 ProteinG TEV LIC cloning vector (2P-T)

Plasmid

#29713

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-ProteinG-TEV

Expression

Bacterial

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par3b

Plasmid

#19388

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 3b (PARD3 Human)

Tags

Myc

Expression

Mammalian

Mutation

Full length, isoform b.

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-Ub KO.G76V

Plasmid

#11932

Depositor

Nico Dantuma

Article

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin KO.G76V (RBX1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Conjugation deficient mutant. All 7 lysines of ub…

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Blast DEST (w524-1)

Plasmid

#19065

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDest-566

Plasmid

#11517

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway Cloning

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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E[beta]C

Plasmid

#24312

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-betacatenin(deltaGSK) // SV40-mCherry (Ctnnb1 Mouse)

Use

Lentiviral

Mutation

Sites phosphorylated by GSK-3beta have been mutat…

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-gateway

Plasmid

#51070

Purpose

gateway adapted mammalian expression vector

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP4B

Plasmid

#116923

Purpose

expresses mCherry-CHMP4B in mammalian cells

Depositor

Sanford Simon

Article

Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

Insert

CHMP4B (CHMP4B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 M

Plasmid

#141274

Purpose

Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Has Service

Cloning Grade DNA

Insert

M (M SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Flag-HA-USP22

Plasmid

#22575

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP22 (USP22 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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