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pLVCT-rtTR-KRAB-2SM2

Plasmid

#11779

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

CAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pLEXSY-hyg2.1_LtIFT43_Flag

Plasmid

#194433

Purpose

Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae

Depositor

Alan Brown

Article

Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.

Insert

Intraflagellar transport complex subunit 43

Use

Leishmania tarentolae expression

Tags

3X Flag and Linker

Promoter

L.donovani promoter elements

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-Rac2(N17)

Plasmid

#11398

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

ras-related C3 botulinum toxin substrate 2 (RAC2 Human)

Tags

YFP

Expression

Mammalian

Mutation

Obtained with Threonine 17 changed to Asparagine.

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX-p47PX(aa2-132)

Plasmid

#19007

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p47phox (NCF1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa2-132 (PX domains)

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
Cherry-ICP0-C3

Plasmid

#134560

Purpose

Expresses Cherry-ICP0 fusion protein

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRS405-FlagPEN2/PS1wt

Plasmid

#207803

Purpose

Yeast integrative vector for expression of FlagPEN2 and Presenilin 1

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits PEN-2 and presenilin1 wt

Expression

Yeast

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pACλCI-β-flap (831-1057)

Plasmid

#53733

Purpose

Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

Depositor

Ann Hochschild

Article

Dove et al Nature. 1997 Apr 10;386(6625):627-30.

Insert

λCI-β-flap fusion

Expression

Bacterial

Promoter

lac UV5

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pRS402-nicastrin/APH-1a-HA

Plasmid

#207804

Purpose

Yeast integrative vector for expression of Nicastrin and APH-1a-HA

Depositor

Dieter Langosch

Article

Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

Insert

Gamma-secretase subunits nicastrin and Aph1a

Expression

Yeast

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB-2SM2

Plasmid

#11652

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB-2SM2, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S2

Plasmid

#128590

Purpose

Expresses tagless S2 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S2 (rpsB E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCZGY3163

Plasmid

#101248

Purpose

GFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

gfp11::rps-18 (rps-18 Nematode, Synthetic)

Use

Gateway

Tags

GFP11

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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MCS_BsmBI

Plasmid

#241170

Purpose

Middle entry vector for mini-Golden subcloning platform; MCS; BsmBI

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

MCS; BsmBI

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pLVPT-rtTR-KRAB

Plasmid

#11777

Purpose

Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

mPGK, GFP, rtTR-KRAB, Tet-off

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-p40PX(aa1-148)

Plasmid

#19008

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p40phox (NCF4 Human)

Tags

GST

Expression

Bacterial

Mutation

aa1-148 (PX domains)

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
p40PX-EYFP

Plasmid

#19011

Depositor

Michael Yaffe

Article

Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Insert

p40phox (NCF4 Human)

Tags

EYFP

Expression

Mammalian

Mutation

aa1-148 (PX domains)

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pEYFP(C1)-PP1alpha

Plasmid

#44231

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

PP1A (PPP1CA Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-SNAP-CLIP-GB1a

Plasmid

#125709

Purpose

Eukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptor

Depositor

Kai Johnsson

Article

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Insert

SNAP-CLIP-GB1a

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET16_Caspase-4_p10

Plasmid

#214308

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_p20_C258A

Plasmid

#214307

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

C258A

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-2mCh-PS

Plasmid

#112268

Purpose

IDOCKS Acceptor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

two copies of mCherry followed by a pseudosubstrate peptide for PKC

Tags

mCh

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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