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pMDC84-SEIPIN3N-1C

Plasmid

#96983

Purpose

Express mutated Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Tags

6xHis and GFP

Expression

Plant

Mutation

A segment of 507 nucleotides from the N terminus…

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC84-SEIPIN1N-3C

Plasmid

#96982

Purpose

Express mutated Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Tags

6xHis and GFP

Expression

Plant

Mutation

A segment of 108 nucleotides from the N terminus…

Promoter

35S

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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LentiCRISPR-sgC17orf89-2

Plasmid

#86135

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

sgRNA targeting C17orf89 (NDUFAF8 )

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA27_no stop

Plasmid

#83860

Purpose

Gateway donor vector for IAA27 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA27 (PAP2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA8_no stop

Plasmid

#83874

Purpose

Gateway donor vector for IAA8 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 8 (IAA8 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA5_no stop

Plasmid

#83868

Purpose

Gateway donor vector for IAA5 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA5 (IAA5 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA3_no stop

Plasmid

#83863

Purpose

Gateway donor vector for IAA3 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA3 (SHY2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA10_no stop

Plasmid

#83848

Purpose

Gateway donor vector for IAA10 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA4_no stop

Plasmid

#83866

Purpose

Gateway donor vector for IAA4 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA4 (ATAUX2-11 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA28_no stop

Plasmid

#83861

Purpose

Gateway donor vector for IAA28 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

NP_568478.1 (IAA28 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA15_no stop

Plasmid

#83853

Purpose

Gateway donor vector for IAA15 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 15 (IAA15 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

Plasmid

#79380

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJK381

Plasmid

#71573

Purpose

Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)

Depositor

T. J. Kappock

Article

Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

Insert

citrate synthase

Expression

Bacterial

Mutation

changes aspartate-317 to glycine

Promoter

recA

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJK382

Plasmid

#71574

Purpose

Produces Thermoplasma acidophilum citrate synthase, Asp317>Asn mutant (TpCS-D317N)

Depositor

T. J. Kappock

Article

Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

Insert

citrate synthase

Expression

Bacterial

Mutation

changes aspartate-317 to asparagine; silent mutat…

Promoter

recA

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSFFV-Cx45

Plasmid

#69808

Purpose

mammalian expression of chick Cx45

Depositor

Richard Veenstra

Article

Veenstra et al Circ Res. 1992 Nov;71(5):1277-83.

Insert

Cx45 (GJC1 Chicken)

Expression

Mammalian

Available Since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only
JW776

Plasmid

#64817

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

N-terminal domain Firefly

Mutation

rCUC2

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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JW777

Plasmid

#64818

Purpose

BiFC CUC3

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

C-terminal domain Firefly

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pIR197

Plasmid

#64819

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

C-terminal domain Firefly

Mutation

rCUC2

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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MT583

Plasmid

#63114

Purpose

The gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 expression in other genetic backgrounds.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

ACD6 (Accelerated Cell Death 6)

Expression

Plant

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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MT79

Plasmid

#63112

Purpose

The artificial microRNA is designed for down-regulating expression of ACD6 mRNA.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

amiR-ACD6

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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