We narrowed to 10,491 results for: fus
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Plasmid#133966PurposeExpresses BE3 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertBE3
UseCRISPR and Synthetic BiologyAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
plko-shmfn2-mfn2-Rescue
Plasmid#160655Purpose"Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"DepositorAvailable SinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB552
Plasmid#74882PurposeGateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB421
Plasmid#74815PurposeGateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB523
Plasmid#74865PurposeGateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Membrane EGFP
Plasmid#198056PurposePlasma membrane localizing EGFP using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane EGFP (LCK Human, Synthetic, A. victoria)
UseSea urchin, mammal, zebrafishTagsEGFPMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mCherry-FKBP-GEF(ARHGEF1)
Plasmid#85152Purposeacute activation of Rho through rapamycin-induced translocation to membrane-localized FRBDepositorInsertFusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)
TagsmCherry-FKBPExpressionMammalianPromoterCAGAvailable SinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS-45
Plasmid#40586DepositorTagsHis-tag and yTAND12ExpressionBacterialPromoterT7Available SinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pQE60 MGH
Plasmid#204244PurposeBacterial expression of a fusion of watermelon glyoxysomal malate dehydrogenase (MDH) and GFP with His tag; Use in CURES (integration of research into undergraduate teaching labs).InsertMGH
Tags6X His and GFPExpressionBacterialMutationfusion of MDH and GFP with His tagPromoterLacAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-I48 ELP
Plasmid#67857PurposeGFP Fluorescent Elastin-like Polypeptide(VPGIG) with 48 repeats containing Isoleucine guest residuesDepositorInsertGFP fused to ELP (VPGIG) repeated 48 times
TagsGFP fused to N-terminal of ELPExpressionMammalianPromoterT7Available SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_KIAA1549_BRAF
Plasmid#205838PurposeExpress mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
ExpressionBacterial and YeastPromoterGAL1Available SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 mRuby3-Rab18
Plasmid#166431PurposeRab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab18
TagsmRuby3ExpressionMammalianPromoterL30 weak promoterAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EnvARGCD
Plasmid#100800Purposeexpress fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domainDepositorInsertfusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
ExpressionMammalianPromoterCAGAvailable SinceDec. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Axin/pCS2HA
Plasmid#16305DepositorAvailable SinceDec. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSPIH6
Plasmid#190676PurposeE. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)DepositorInsertsSmall ubiquitin-like modifier
Mxe GyrA intein with C-terminal chitin binding domain and His tag
TagsChitin binding domain, His tag, and N-terminal Hi…ExpressionBacterialMutationAdded C-terminal his tag and NonePromoterT7Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-mScarlet
Plasmid#191098PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-mScarlet
UseAAV and Synthetic BiologyTagsThree repeats of the nuclear localizzation seque…ExpressionMammalianMutationOptimized to human codon usagePromoterCMV enhancer and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
(271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)
Plasmid#168102PurposeHA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nEPEA-(EAAAK)3-OGA(544-706)
TagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
R703-M72-303: CMV51p> Halotag-Hs.KRAS4b
Plasmid#159679PurposeMammalian protein expression of KRAS4b-Halotag fusionDepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
Ana GvpC-SpyTag-His in pET28a
Plasmid#85737PurposeExpresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coliDepositorInsertGas vesicle protein C (fused to SpyTag and His-tagged)
TagsHis-tag and SpyTagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available SinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only