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We narrowed to 7,280 results for: gnal

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Showing: 7201 - 7220 of 7280 results

  1. pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid
    #181855
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
    Promoter
    CMV
    Available Since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181855-plasmid-map-sequence-id-362798

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  3. ST-2xTstem(5A)-GFP

    Plasmid
    #162504
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162504-plasmid-map-sequence-id-323646

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  4. ST-2xTstem-GFP

    Plasmid
    #162503
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162503-plasmid-map-sequence-id-323511

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  5. GFP-Cstem-Tac(5A)

    Plasmid
    #162497
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a stem region and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five threonine residues are mutated into alan…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162497-plasmid-map-sequence-id-323484

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  6. GFP-Tac-STC(5A)

    Plasmid
    #162491
    Purpose
    Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Tac is in partial length with its stem region, TM…
    Available Since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162491-plasmid-map-sequence-id-323469

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  10. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Amy Palmer
    Article
    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pEN_TT 3XFLAG-S37A-GATA6-3XAU1_RNAi resistant

    Plasmid
    #72924
    Purpose
    Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged, RNAi resistant human S37A-GATA6
    Depositor
    Kevin Janes
    Article
    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:
    Insert
    GATA Binding Protein 6 (GATA6 Human)
    Use
    Gateway Cloning
    Tags
    3XAU1 and 3XFLAG
    Expression
    Bacterial
    Mutation
    Serine#37 changed to Alanine;Silent mutations mad…
    Promoter
    TRE tight
    Available Since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    72924-plasmid-map-sequence-id-138470

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Showing: 7201 - 7220 of 7280 results