We narrowed to 16,540 results for: puromycin

1. pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

   Plasmid

   #183951

   Purpose

   Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

   Depositor

   Eugene Yeo

   Article

   Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pABpuro-BluF

   Plasmid

   #46824

   Purpose

   luciferase reporter for BmalI promoter

   Depositor

   Steven Brown

   Article

   Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

   Insert

   Bmal1 (Bmal1 Mouse)
   

   Use

   Lentiviral and Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Bmal1

   Available Since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-ATF4 mScarlet NLS

   Plasmid

   #115969

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Human, Synthetic)
   

   Use

   Lentiviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAGGS-EGFP

   Plasmid

   #22212

   Purpose

   AAV zinc finger donor vector for genomic targeting

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   CAGGS-eGFP
   

   Use

   Zfn donor

   Available Since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFR L858R

   Plasmid

   #11012

   Purpose

   Retroviral construct for expressing human EGFR with L858R mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR L858R (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   L858R (tumor-derived)

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

   Plasmid

   #183790

   Purpose

   A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Inserts

   P2A-eGFP-TargetBC-5xTAPE-1
   pegRNA-InsertBC
   

   Use

   Synthetic Biology

   Mutation

   G>A in the gRNA scaffold- please see depositor…

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p7056 pHAGE-P-CMVt-N-HA-GAW-CPT1A

   Plasmid

   #100146

   Purpose

   Expresses CPT1A

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   CPT1A (CPT1A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF2895

   Plasmid

   #144355

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MTA1 (MTA1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

   Plasmid

   #207832

   Purpose

   telomere specific nuclease to create double-strand breaks at telomeres

   Depositor

   Roger Greenberg

   Article

   Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag, DD, ER, mCherry and FokI

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

    Plasmid

    #218528

    Purpose

    sgRNA targeting human EIF2AK4/GCN2

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK4 (EIF2AK4 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Tet-OsTIR1(F74G)-V5

    Plasmid

    #158664

    Purpose

    Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(F74G) AAVS1
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    F74G

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEFIRES-P-ACSL3-mCherry

    Plasmid

    #87158

    Purpose

    Fluorescent protein targeted to lipid droplets (LDs) from the endoplasmic reticulum (ER)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ACSL3 (ACSL3 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF-1-alpha

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-dual-CRISPR-U6-H1(pBP43)

    Plasmid

    #218930

    Purpose

    The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    human U6 and H1

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-HIF1alpha-wt-pBabe-puro

    Plasmid

    #19365

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt human cDNA

    Available Since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBMN_HA-TBK1 (kinase dead - D135N)

    Plasmid

    #199206

    Purpose

    Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    D135N

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TLCV2-Scal-T2A-EGFP-Puro

    Plasmid

    #234949

    Purpose

    Doxycycline-inducible mitochondria-localized ScaI expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    ScaI restriction enzyme with N-terminal COX8 mitochondrial localization signal
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. TLCV2-ApaLI-T2A-EGFP-Puro

    Plasmid

    #234948

    Purpose

    Doxycycline-inducible mitochondria-localized ApaLI expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    ApaLI restriction enzyme with N-terminal COX8 mitochondrial localization signal
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGK-DTAG-NTERM-GFP

    Plasmid

    #185775

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 10279-M28-663

    Plasmid

    #225662

    Purpose

    Lentivirus protein expression. Matched control construct for 8522-M01-663 (Addgene # 225660) gives basal expression from mCMVp for gating/thresholding

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCopGFP
    

    Use

    Lentiviral

    Promoter

    mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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