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BB1_23

Plasmid

#98496

Purpose

empty BB1 for cds cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Publication

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR P4-P1R-EYFP

Plasmid

#48350

Purpose

Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

Depositor

Anna Planas, Tomas Santalucia

Publication

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

EYFP

Use

Gateway Cloning

Mutation

Contains a Kozak sequence. Does not contain a sto…

Promoter

none

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

Plasmid

#207880

Purpose

AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

Depositor

Yeh-Hsing Lao

Publication

Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLIX403-EBFP2-ccdB-Puro

Plasmid

#158552

Purpose

Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Lentiviral

Tags

EBFP2

Expression

Mammalian

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-DPP4

Plasmid

#158439

Purpose

Gateway Cloning compatible entry vector for the human DPP4 gene.

Depositor

Alejandro Chavez, Sho Iketani

Insert

DPP4 (DPP4 Human)

Use

Gateway Cloning

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pHBJT017

Plasmid

#225168

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (AmpR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT024

Plasmid

#225175

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (SmR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT019

Plasmid

#225170

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (TetR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT020

Plasmid

#225171

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (TetR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT022

Plasmid

#225173

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (GmR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT016

Plasmid

#225167

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (ChlR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT015

Plasmid

#225166

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (ChlR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT018

Plasmid

#225169

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (AmpR).

Depositor

Jenny-Lee Thomassin

Publication

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pJAT32

Plasmid

#204297

Purpose

pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.

Depositor

David Stern

Publication

Stern et al bioRxiv 2023.06.17.545412

Type

Empty backbone

Use

CRISPR

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGS-21a-PcAad1

Plasmid

#163039

Purpose

expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

Depositor

Jean-Marie Francois

Publication

Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

Insert

aryl-alcohol dehydrogenase

Tags

GST and His6

Expression

Bacterial

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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KK402: pMVP/Zeo-DEST

Plasmid

#121859

Purpose

pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Expression

Mammalian

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-Tdtomato-iRFP670-GFP

Plasmid

#111815

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Publication

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Tdtomato
iRFP670
GFP

Use

Cloning intermediate

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-H11r1-2-DD-Cas9

Plasmid

#85978

Purpose

Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

Depositor

Michele Calos

Publication

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

DD-SpCas9

Use

CRISPR

Tags

FKBP12 L106P DD

Expression

Mammalian

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRL2697

Plasmid

#70693

Purpose

pDU1-E. coli shuttle vector (Cm-r Em-r) with extended polylinker

Depositor

Peter Wolk

Publication

Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

Type

Empty backbone

Use

Bacterial shuttle vector

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pNH051

Plasmid

#42157

Depositor

Colin Dolphin

Publication

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

GFP-RT-GFP

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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