We narrowed to 8,460 results for: gnal
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Plasmid#82613PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)Mutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYSD313
Plasmid#212931PurposeEncodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertSed1 (SED1 Budding Yeast)
ExpressionYeastAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pMXs-IG
Plasmid#51258PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR and RetroviralTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
SSTR2-DuET
Plasmid#213377PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.RGS4.C-HA
Plasmid#154847PurposeExpresses murine RGS4 protein with HA-tag at C-terminusDepositorInsertRegulator of G protein Signaling 4 (Rgs4 Mouse)
UseAAVTagsHA-tagExpressionMammalianPromoterCMVAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV2/9-CW3SL-mCherry-DrTrkB
Plasmid#184002PurposeAAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherryDepositorAvailable SinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL
Plasmid#65712PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL)DepositorInsert7xTcf, minCMV, 3xNLS, CNL
UseLuciferaseAvailable SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFUW-TetO-mCherry-cNLS
Plasmid#138531PurposeLentiviral inducible expression of mCherry with nuclear localization signalDepositorInsertmCherry
UseLentiviralTags3x SV40 cNLSExpressionMammalianPromoterTRE-CMVAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJR021
Plasmid#198814PurposeVariant of yellow fluorescent protein for transient gene expressionDepositorInsert15xUAS::destabilizedYFP::let-838 3'UTR
ExpressionWormMutationYFP with destabilized signal from mouse ornithine…Available SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-B8
Plasmid#135512PurposeMammalian expression of myc-tagged HLA-B*08:01DepositorInsertHLA-B*08:01 (HLA-B Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Sa-dCas9-NLS-3xFLAG/pcDNA3.1
Plasmid#98041PurposeExpresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interestDepositorInsertSa-dCas9-NLS-3xFLAG
UseCRISPRTags3xFLAG tag and NLS (nuclear localization signal)ExpressionMammalianMutationD10A + H557APromoterCMVAvailable SinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-H2-Dd
Plasmid#135518PurposeMammalian expression of myc-tagged H2-DdDepositorInsertH2-Dd (H2-D1 Mouse)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationC121SPromoterCMVAvailable SinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cTRb413
Plasmid#188261PurposePlasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_miRFP703
Plasmid#183759PurposeBxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703DepositorInsertmiRFP703
TagsLck lipidation signalExpressionMammalianAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Th3
Plasmid#188064PurposePROSS-designed high-redox potential laccase from Trametes hirsutaDepositorInsertPROSS-designed high-redox potential laccase from Trametes hirsuta
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 mutations, described in publicationAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectPromoterPolyhedrinAvailable SinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
TagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXa3
TagsmCherryExpressionBacterial, Insect, and Mamm…PromoterChicken B-ActinAvailable SinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEX-CMV-SP-CFP-STM2(15-746)
Plasmid#18863DepositorInsertStromal Interaction Molecule 2 (STIM2 Human)
TagsCFPExpressionMammalianMutationCFP inserted between signal peptide and residues …Available SinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Fla
Plasmid#84866PurposeBaculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Fla
UseBaculovirusExpressionInsectPromoterPolyhedrinAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only