We narrowed to 8,324 results for: gnal

1. pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

   Plasmid

   #199447

   Purpose

   Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

   Depositor

   Baohui Chen

   Article

   Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

   Insert

   dSaCas9-10xGCN4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C (UBC)

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHW491

   Plasmid

   #198808

   Purpose

   In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::Tetx cDNA::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJL049

   Plasmid

   #198809

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP6s-SL2-mKate2::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-T Easy-Cas9-nls-CDS1

   Plasmid

   #160231

   Purpose

   Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
   

   Use

   Level 0 of moclo golden gate position cds1

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2

   Plasmid

   #186413

   Purpose

   Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   lacZ (lacZ E. coli)
   

   Use

   Gateway destination vector

   Tags

   Nuclear localization signal

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3_HLA-cmyc-GRM3 C127A TMD Y616M

   Plasmid

   #140454

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and Y616M mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, tyrosine 616 to methioni…

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3_HLA-cmyc-GRM3 C127A TMD L624N

   Plasmid

   #140455

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and L624N mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, leucine 624 to asparagine

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3_HLA-cmyc-GRM3 C127A TMD A634N

   Plasmid

   #140456

   Purpose

   Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and A634N mutant

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine, alanine 634 to asparagine

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4_HLA-flag-GRM3 C127A-VN

   Plasmid

   #140449

   Purpose

   Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)

   Depositor

   Erik Procko

   Insert

   GRM3 (GRM3 Human)
   

   Use

   Tags

   FLAG tag, Signal/leader sequence from HLA class I…

   Expression

   Mammalian

   Mutation

   Cysteine 127 to alanine

   Promoter

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid

    #140450

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine

    Promoter

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid

    #140452

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, isoluecine 578 to alanine

    Promoter

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3_HLA-cmyc-GRM3 C127A TMD F588V

    Plasmid

    #140453

    Purpose

    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and F588V mutant

    Depositor

    Erik Procko

    Insert

    GRM3 (GRM3 Human)
    

    Use

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    Cysteine 127 to alanine, phenylalanine 588 to val…

    Promoter

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-MR1 (M50P)

    Plasmid

    #178651

    Purpose

    Mammalian expression of myc-tagged MR1 (M50P)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    MR1 (MR1 Human)
    

    Use

    Tags

    Extracellular c-myc epitope ta and Signal peptide…

    Expression

    Mammalian

    Mutation

    M50P

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4 MR1 (H83P)

    Plasmid

    #178652

    Purpose

    Mammalian expression of myc-tagged MR1 (H83P)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    MR1 (MR1 Human)
    

    Use

    Tags

    Extracellular c-myc epitope ta and Signal peptide…

    Expression

    Mammalian

    Mutation

    H83P

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCEP4 MR1 (Q153P)

    Plasmid

    #178653

    Purpose

    Mammalian expression of myc-tagged MR1 (Q153P)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    MR1 (MR1 Human)
    

    Use

    Tags

    Extracellular c-myc epitope ta and Signal peptide…

    Expression

    Mammalian

    Mutation

    Q153P

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTS1046-Tier1-PhCMV-4xCox8-mRuby2

    Plasmid

    #169537

    Purpose

    Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

    Plasmid

    #182305

    Purpose

    E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

    Depositor

    Andrew York

    Article

    Seidel et al (2021), doi: 10.5281/zenodo.5810930

    Insert

    pH-Countdown insertion into GECO 1.2 calcium sensing domains
    

    Use

    Tags

    A modified TorA periplasmic targeting sequence pl…

    Expression

    Bacterial

    Mutation

    Spontaneous mutation of calmodulin's penulti…

    Promoter

    t7

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-hPPP3R2

    Plasmid

    #179163

    Purpose

    Expression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-mPpp3r2

    Plasmid

    #179161

    Purpose

    Expression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-mSpata33 (I81A)-PA

    Plasmid

    #179131

    Purpose

    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (Spata33 Mouse)
    

    Use

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Changed Isoleucine 81 to Alanine

    Promoter

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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