We narrowed to 7,572 results for: Lif
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Plasmid#52879PurposeEncodes human Connexin 43 resistant to siRNA with Serine to Alanine mutation at 373DepositorInsertGJA1 (GJA1 Human)
UseTagsExpressionMammalianMutationSerine 373 mutated to AlalninePromoterCMVAvailable sinceMay 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN-CAG-mRFP-PCNA pc2729
Plasmid#166040PurposeExpresses mRFP tagged human PCNA in mammalian cells.DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-HP1α-miRFP670-Cry2WT
Plasmid#122442PurposeExpresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertHP1 alpha (CBX5 Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
beta catenin binding mutant of alpha catenin sensor
Plasmid#71711PurposeThis form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctionsDepositorInsertalpha-catenin Δβ-cat mutant FRET Sensor
UseTagsECFP and YPETExpressionMammalianMutationDeleted amino acid residues 48- 163PromoterCMVAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cer(1-158)-RGS7
Plasmid#55760PurposeAn amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorInsertmCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
UseTagsCer(1-158) was fused to the amino terminus of RGS…ExpressionMammalianMutationRGS7 was amplified via PCR, which added an N-term…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p2rbs-GST-Ring1b(1-159)-Bmi1(1-109)
Plasmid#63139PurposeExpression of murine Ring 1b (1-159 aa) and Bmi1 (1-109 aa)DepositorUseTagsGSTExpressionBacterialMutationResidues 1-109 and Residues 1-159PromotertacAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGV3319
Plasmid#170277PurposeExpression of mutant Bst polymerase (Bst-LF D720A) for loop mediated isothermal amplification (LAMP)DepositorInsertBst-LF D720A
UseTagsHexahistidineExpressionBacterialMutationD720APromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR26-mBMP7
Plasmid#127374PurposeAllows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorInsertBMP7 (Bmp7 Mouse)
UseCRISPR and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 HA D122A pcDNA3
Plasmid#206108Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1DepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationD122APromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR P4-P1R-EGFP
Plasmid#48348PurposeContributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationContains a Kozak sequence. Does not contain a sto…PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO SnRK2.2 (20-362)
Plasmid#177845PurposeBacterial Expression of SnRK2.2DepositorInsertSNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)
UseTags6xHis-SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARF4-GFP
Plasmid#67392PurposeMammalian expression of hArf4 with C-term GFP tagDepositorInsertARF4 (ARF4 Human)
UseTagsGFPExpressionMammalianMutationV53A, L123P and M134I mutations were unintended P…PromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3
Plasmid#48811PurposeThis report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.DepositorInsertnAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
UseTagsExpressionMammalianMutation*PromoterCMVAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS
Plasmid#59388PurposeDestination vector with RBPMS for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceFeb. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-NoStart
Plasmid#49853PurposeEncodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationStart codon mutated to from methionine to isoleuc…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-INS-904
Plasmid#53970Purpose904 bp insert from human INS gene used as a control for methylation-specific PCR assaysDepositorInserthuman INS gene partial (INS Human)
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBI121::chitGFP5:AQ
Plasmid#240450PurposeExpression of indicator protein fusion (soluble modified GFP5 & Aequorin) for monitoring calcium concentrations in cell walls of higher plants. For details, see Comments.DepositorInsertFusion of Chitinase signal, smGFP5, and Aequorin
UseTagsExpressionBacterial and PlantMutationGFP5 for expression plants (PMID 9122158); Solubl…PromoterCauliflower mosaic virus 35S promoter (GenBank AJ…Available sinceSept. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
Mirror GFP[ENE(WT)-mascRNA](pAVA2965)
Plasmid#239351PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.DepositorInsertMALAT1 ENE(WT)-mascRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Mirror RFP[ENE(WT)-mascRNA](pAVA2987)
Plasmid#239348PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.DepositorInsertMALAT1 ENE(WT)-mascRNA
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
TOPO-LG4_chr5:550kb antisense
Plasmid#205468PurposeEnhancer containing plasmid used for assessing enhancer::promoter interactionsDepositorInsertchr5:550kb LG4
UseSynthetic Biology; Subcloning of taq-amplified pc…TagsExpressionMutationPromoterAvailable sinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only