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Addgene

We narrowed to 7,572 results for: Lif

Showing: 7261 - 7280 of 7572 results
  1. beta catenin binding mutant of alpha catenin sensor

    Plasmid
    #71711
    Purpose
    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions
    Depositor
    Insert
    alpha-catenin Δβ-cat mutant FRET Sensor
    Use
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 48- 163
    Promoter
    CMV
    Available since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Insert
    mCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pR26-mBMP7

    Plasmid
    #127374
    Purpose
    Allows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Insert
    BMP7 (Bmp7 Mouse)
    Use
    CRISPR and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pDONR P4-P1R-EGFP

    Plasmid
    #48348
    Purpose
    Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Insert
    EGFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a Kozak sequence. Does not contain a sto…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pCDNA3.1-nAChr-beta3

    Plasmid
    #48811
    Purpose
    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.
    Insert
    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    *
    Promoter
    CMV
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA3.2-Cx43-NoStart

    Plasmid
    #49853
    Purpose
    Encodes human connexin 43 with the start methionine codon mutated to isoleucine and with wobble mutations conferring resistance against an siRNA
    Depositor
    Insert
    Connexin 43 (GJA1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Start codon mutated to from methionine to isoleuc…
    Promoter
    CMV
    Available since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pBI121::chitGFP5:AQ

    Plasmid
    #240450
    Purpose
    Expression of indicator protein fusion (soluble modified GFP5 & Aequorin) for monitoring calcium concentrations in cell walls of higher plants. For details, see Comments.
    Depositor
    Insert
    Fusion of Chitinase signal, smGFP5, and Aequorin
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    GFP5 for expression plants (PMID 9122158); Solubl…
    Promoter
    Cauliflower mosaic virus 35S promoter (GenBank AJ…
    Available since
    Sept. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. Mirror GFP[ENE(WT)-mascRNA](pAVA2965)

    Plasmid
    #239351
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.
    Depositor
    Insert
    MALAT1 ENE(WT)-mascRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. Mirror RFP[ENE(WT)-mascRNA](pAVA2987)

    Plasmid
    #239348
    Purpose
    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.
    Depositor
    Insert
    MALAT1 ENE(WT)-mascRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 7261 - 7280 of 7572 results